MEKK2 Antibody (722728)

R&D Systems | Catalog # MAB7128

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 722728
View all MEKK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MEKK2
Asn42-Tyr264
Accession # Q9Y2U5

Specificity

Detects recombinant human MEKK2 in direct ELISA and human, mouse, rat MEKK2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEKK1, 3, or 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MEKK2 Antibody (722728)

Detection of Human, Mouse, and Rat MEKK2 antibody by Western Blot.

Detection of Human, Mouse, and Rat MEKK2 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Saos-2 human osteosarcoma cell line, BJAB human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MEKK2 Monoclonal Antibody (Catalog # MAB7128) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MEKK2 antibody in 293T Human Cell Line by Immunocytochemistry (ICC).

MEKK2 in 293T Human Cell Line.

MEKK2 was detected in immersion fixed 293T human embryonic kidney cell line using Mouse Anti-Human/Mouse/Rat MEKK2 Monoclonal Antibody (Catalog # MAB7128) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse or rat samples.

Applications for MEKK2 Antibody (722728)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed 293T human embryonic kidney cell line

Western Blot

2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line, Saos‑2 human osteosarcoma cell line, BJAB human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB7128 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MEKK2

MAPK/ERK kinase kinase 2 (MEKK2) is a widely expressed 70 kDa member of the MEKK/STE11 subfamily of Ser/Thr kinases. It contains a PB1 domain (aa 43-122) which mediates dimerization with MEK5, a region with several phosphorylation sites, and a protein kinase domain (aa 357-617) that includes a site for activating phosphorylation at Thr524. Within aa 42-264, human MEKK2 shares 93% aa sequence identity with mouse and rat MEKK2. Activated MEKK2 translocates from the cytosol to the nucleus, where it forms a complex that binds and activates MKK7 in the c-Jun N-terminal kinase (JNK) pathway, relaying mitogenic and stress signals.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 2

Alternate Names

MAP3K2, MAPKKK2

Entrez Gene IDs

10746 (Human); 26405 (Mouse); 171492 (Rat)

Gene Symbol

MAP3K2

UniProt

Q9Y2U5

Additional MEKK2 Products

Product Documents for MEKK2 Antibody (722728)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for MEKK2 Antibody (722728)

For research use only

Related Research Areas

Intracellular Kinases
NFkB Pathway

Customer Reviews for MEKK2 Antibody (722728) (1)

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  • Human/Mouse/Rat MEKK2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 11/30/2021
    MEKK2 Antibody (722728) MAB7128

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Protocols

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