Human/Mouse/Rat Neurofascin Antibody

Catalog # Availability Size / Price Qty
MAB3235
MAB3235-SP
Detection of Human, Mouse, and Rat Neurofascin by Western Blot.
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Citations (1)
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Human/Mouse/Rat Neurofascin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects rat Neurofascin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 727030
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neurofascin
Ile25-Ala1031
Accession # NP_446361
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Neurofascin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Neurofascin by Western Blot. Western blot shows lysates of rat brain tissue, mouse brain tissue, human brain (cortex) tissue, human brain (cerebellum) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Neurofascin Monoclonal Antibody (Catalog # MAB3235) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Neurofascin at approximately 140 to 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurofascin

Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).

References
  1. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  2. Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
  3. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  4. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  5. Collinson, J.M. et al. (1998) Glia 23:11.
  6. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  7. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  8. Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
  9. Sherman, D.L. et al. (2005) Neuron 48:737.
  10. Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
  11. Charles, P. et al. (2002) Curr. Biol. 12:217.
  12. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  13. Maier, O. et al. (2006) Exp. Cell Res. 312:500.
Entrez Gene IDs
23114 (Human); 269116 (Mouse); 116690 (Rat)
Alternate Names
DKFZp686P2250; KIAA0756FLJ46866; neurofascin homolog (chicken); neurofascin homolog; Neurofascin; NF; NFASC; NRCAML

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Citation for Human/Mouse/Rat Neurofascin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay
    Authors: Katsunori Ikewaki
    J. Neuroimmunol., 2016;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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