Human/Mouse/Rat Neurofascin Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat Neurofascin by Western Blot.
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Product Details
Citations (9)
Supplemental Products

Human/Mouse/Rat Neurofascin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, rat Neurofascin in Western blots and rat Neurofascin in direct ELISAs.
Polyclonal Chicken IgY
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant rat Neurofascin
Accession # NP_446361
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Neurofascin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Neurofascin by Western Blot. Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. Specific bands were detected for Neurofascin at approximately 140 and186 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neurofascin antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Neurofascin in Rat Brain. Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (yellow; Catalog # NL016) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and Schwann cells (perinodal regions). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurofascin

Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).

  1. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  2. Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
  3. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  4. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  5. Collinson, J.M. et al. (1998) Glia 23:11.
  6. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  7. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  8. Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
  9. Sherman, D.L. et al. (2005) Neuron 48:737.
  10. Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
  11. Charles, P. et al. (2002) Curr. Biol. 12:217.
  12. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  13. Maier, O. et al. (2006) Exp. Cell Res. 312:500.
Entrez Gene IDs
23114 (Human); 269116 (Mouse); 116690 (Rat)
Alternate Names
DKFZp686P2250; KIAA0756FLJ46866; neurofascin homolog (chicken); neurofascin homolog; Neurofascin; NF; NFASC; NRCAML

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Citations for Human/Mouse/Rat Neurofascin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Distinct Changes in Calpain and Calpastatin during PNS Myelination and Demyelination in Rodent Models
    Authors: JA Miller, DE Drouet, LM Yermakov, MS Elbasiouny, FZ Bensabeur, M Bottomley, K Susuki
    International Journal of Molecular Sciences, 2022;23(23):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells
    Authors: G Della-Flor, ER Wilson, LN Marziali, E Hurley, N Silvestri, B He, BW O'Malley, B Beirowski, Y Poitelon, L Wrabetz, ML Feltri
    Nature Communications, 2021;12(1):3285.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells
    Authors: KJ Chang, I Agrawal, A Vainshtein, WY Ho, W Xin, G Tucker-Kel, K Susuki, E Peles, SC Ling, JR Chan
    Elife, 2021;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Immunoadsorption and Plasma Exchange in Seropositive and Seronegative Immune-Mediated Neuropathies
    Authors: AJ Davies, J Fehmi, M Senel, H Tumani, J Dorst, S Rinaldi
    J Clin Med, 2020;9(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Accumulation of Neurofascin at nodes of Ranvier is regulated by a Paranodal Switch
    Authors: Y Zhang, S Yuen, E Peles, JL Salzer
    J. Neurosci., 2020;0(0):.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Glial ?II spectrin contributes to paranode formation and maintenance
    Authors: K Susuki, DR Zollinger, KJ Chang, C Zhang, CY Huang, CR Tsai, MR Galiano, Y Liu, SD Benusa, LM Yermakov, RB Griggs, JL Dupree, MN Rasband
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Methylglyoxal Disrupts Paranodal Axoglial Junctions via Calpain Activation
    Authors: RB Griggs, LM Yermakov, DE Drouet, DVM Nguyen, K Susuki
    ASN Neuro, 2018;10(0):1759091418766.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. The paranodal cytoskeleton clusters Na(+) channels at nodes of Ranvier
    Authors: V Amor, C Zhang, A Vainshtein, A Zhang, DR Zollinger, Y Eshed-Eise, PJ Brophy, MN Rasband, E Peles
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy
    Authors: L Querol, AM Siles, R Alba-Rovir, A Jáuregui, J Devaux, C Faivre-Sar, J Araque, R Rojas-Garc, J Diaz-Maner, E Cortés-Vic, G Nogales-Ga, M Navas-Madr, E Gallardo, I Illa
    Sci Rep, 2017;7(1):14411.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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