Human/Mouse/Rat Neurofascin Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, rat Neurofascin in Western blots and rat Neurofascin in direct ELISAs.
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat Neurofascin
    Ile25-Ala1031
    Accession # NP_446361
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Neurofascin by Western Blot. Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. Specific bands were detected for Neurofascin at approximately 140 and186 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Neurofascin in Rat Brain. Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (yellow; Catalog # NL016) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and Schwann cells (perinodal regions). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurofascin

Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).

  • References:
    1. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
    2. Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
    3. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
    4. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
    5. Collinson, J.M. et al. (1998) Glia 23:11.
    6. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
    7. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
    8. Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
    9. Sherman, D.L. et al. (2005) Neuron 48:737.
    10. Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
    11. Charles, P. et al. (2002) Curr. Biol. 12:217.
    12. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
    13. Maier, O. et al. (2006) Exp. Cell Res. 312:500.
  • Entrez Gene IDs:
    23114 (Human); 269116 (Mouse); 116690 (Rat)
  • Alternate Names:
    DKFZp686P2250; KIAA0756FLJ46866; neurofascin homolog (chicken); neurofascin homolog; Neurofascin; NF; NFASC; NRCAML
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The paranodal cytoskeleton clusters Na(+) channels at nodes of Ranvier
    Authors: V Amor, C Zhang, A Vainshtein, A Zhang, DR Zollinger, Y Eshed-Eise, PJ Brophy, MN Rasband, E Peles
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Isotype Controls
Description Application Cat# Citations Images  

Normal Chicken IgY Control

Ctrl AB-101-C 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat Anti-Chicken IgY NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL016 1
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Goat Anti-Chicken IgY NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL017 1
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Goat Anti-Chicken IgY Antibody

WB AF010 1
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Goat Anti-Chicken IgY NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL018
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Goat Anti-Chicken IgY Biotinylated Antibody

WB BAF010
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