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Human/Mouse/Rat nNOS Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB24162S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB24162S-100UG

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Human/Mouse/Rat nNOS Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human nNos in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 85327
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
Ser218-Ser1434
Accession # P29475
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: nNOS

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. nNOS exists as homodimers containing a cytochrome P450‑like prosthetic heme group in the N-terminal half. It also has a tightly bound FAD and FMN group in the C-terminal half. At least 4 isoforms of human nNOS are known. Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. It also shares 96% sequence identity with mouse or rat nNOS.

Long Name
Neuronal Nitric Oxide Synthase
Entrez Gene IDs
4842 (Human); 18125 (Mouse)
Alternate Names
bNOS; brain; Constitutive NOS; EC 1.14.13.39; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1

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