Human/Mouse/Rat Nucleostemin Alexa Fluor® 532-conjugated Antibody

Catalog #: AF1638X Datasheet / COA / SDS

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AF1638X-100UG

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Human/Mouse/Rat Nucleostemin Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, rat Nucleostemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat Nucleostemin C-terminal peptide (aa 281-538) is observed. Rat Nucleostemin specific IgG was purified by fi

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat Nucleostemin
Lys2-Ile538
Accession # Q811S9

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nucleostemin

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells (1-3).

Entrez Gene IDs

26354 (Human); 30877 (Mouse); 290556 (Rat)

Alternate Names

C77032; E2IG3NNP47; estradiol-induced nucleotide binding protein; GNL3; guanine nucleotide binding protein-like 3 (nucleolar); guanine nucleotide-binding protein-like 3; MGC800; Novel nucleolar protein 47; NSE2-induced gene 3 protein; Nucleolar GTP-binding protein 3; Nucleostemin