Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody Summary
Complete Your Research
Complete Your Experiment
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse and Human Phospho-CDC2 (Y15) by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 0.2 µg/mL nocodazole or 12 µM aphidicolin or 1 mM hydroxurea for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDC2/CDK1
CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent Kinase 1), is a member of the CDK family of serine/threonine kinases. The CDKs are important regulators of cell cycle progression and their activities are largely controlled by association with Cyclins, and activating and inhibitory phosphorylations. Entry into mitosis is initiated by CDC2. Full activation of CDC2 requires phosphorylation at T161 and association with CyclinB. In contrast, phosphorylation of CDC2 at Y15 and T14 during the G2-phase of the cell cycle inhibits activity, and dephosphorylation of Y15 and T14 by CDC25 phosphatase during late G2 restores activity.
Product Datasheets
Citations for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement
Authors: J Liu, Y Wang, C Mu, M Li, K Li, S Li, W Wu, L Du, X Zhang, C Li, W Peng, J Shen, Y Liu, D Yang, K Zhang, Q Ning, X Fu, Y Zeng, Y Ni, Z Zhou, Y Liu, Y Hu, X Zheng, T Wen, Z Li, Y Liu
Nature Communications, 2022;13(1):4308.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
Haematologica, 2017;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Unique functions of CHK1 and WEE1 underlie synergistic anti-tumor activity upon pharmacologic inhibition.
Authors: Guertin, Amy D, Martin, Melissa, Roberts, Brian, Hurd, Melissa, Qu, Xianlu, Miselis, Nathan R, Liu, Yaping, Li, Jing, Feldman, Igor, Benita, Yair, Bloecher, Andrew, Toniatti, Carlo, Shumway, Stuart D
Cancer Cell Int, 2012;12(1):45.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The p75 neurotrophin receptor interacts with multiple MAGE proteins.
Authors: Tcherpakov M, Bronfman FC, Conticello SG, Vaskovsky A, Levy Z, Niinobe M, Yoshikawa K, Arenas E, Fainzilber M
J. Biol. Chem., 2002;277(51):49101-4.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody and earn rewards!
Have you used Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
