Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse and Human Phospho-CDC2 (Y15) by Western Blot.
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Product Details
Citations (4)
Supplemental Products

Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat CDC2 when phosphorylated at Y15.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing CDC2 Y15 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.2 µg/mL
See below
5-15 µg/mL
Immersion fixed MCF-7 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Human Phospho-CDC2 (Y15) antibody by Western Blot. View Larger

Detection of Mouse and Human Phospho-CDC2 (Y15) by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 0.2 µg/mL nocodazole or 12 µM aphidicolin or 1 mM hydroxurea for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDC2/CDK1

CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent Kinase 1), is a member of the CDK family of serine/threonine kinases. The CDKs are important regulators of cell cycle progression and their activities are largely controlled by association with Cyclins, and activating and inhibitory phosphorylations. Entry into mitosis is initiated by CDC2. Full activation of CDC2 requires phosphorylation at T161 and association with CyclinB. In contrast, phosphorylation of CDC2 at Y15 and T14 during the G2-phase of the cell cycle inhibits activity, and dephosphorylation of Y15 and T14 by CDC25 phosphatase during late G2 restores activity.

Long Name
Cell Division Cycle 2/Cyclin-dependent Kinase 1
Entrez Gene IDs
983 (Human); 12534 (Mouse); 54237 (Rat)
Alternate Names
CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC; EC; G1 to S and G2 to M; p34 protein kinase; P34CDC2

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Citations for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement
    Authors: J Liu, Y Wang, C Mu, M Li, K Li, S Li, W Wu, L Du, X Zhang, C Li, W Peng, J Shen, Y Liu, D Yang, K Zhang, Q Ning, X Fu, Y Zeng, Y Ni, Z Zhou, Y Liu, Y Hu, X Zheng, T Wen, Z Li, Y Liu
    Nature Communications, 2022;13(1):4308.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
    Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
    Haematologica, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Unique functions of CHK1 and WEE1 underlie synergistic anti-tumor activity upon pharmacologic inhibition.
    Authors: Guertin, Amy D, Martin, Melissa, Roberts, Brian, Hurd, Melissa, Qu, Xianlu, Miselis, Nathan R, Liu, Yaping, Li, Jing, Feldman, Igor, Benita, Yair, Bloecher, Andrew, Toniatti, Carlo, Shumway, Stuart D
    Cancer Cell Int, 2012;12(1):45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. The p75 neurotrophin receptor interacts with multiple MAGE proteins.
    Authors: Tcherpakov M, Bronfman FC, Conticello SG, Vaskovsky A, Levy Z, Niinobe M, Yoshikawa K, Arenas E, Fainzilber M
    J. Biol. Chem., 2002;277(51):49101-4.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot


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