Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

R&D Systems | Catalog # AF888

R&D Systems
Discontinued Product
AF888 has been discontinued. View all CDC2/CDK1 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CDC2/CDK1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing CDC2 Y15 site

Specificity

Detects human, mouse, and rat CDC2 when phosphorylated at Y15.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

Detection of Mouse and Human Phospho-CDC2 (Y15) antibody by Western Blot.

Detection of Mouse and Human Phospho-CDC2 (Y15) by Western Blot.

Western blot shows lysates of L-929 mouse fibroblast cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 0.2 µg/mL nocodazole or 12 µM aphidicolin or 1 mM hydroxurea for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human CDC2/CDK1 by Western Blot

Detection of Human CDC2/CDK1 by Western Blot

Cooperative induction of DNA damage in vivo by WEE1 and CHK1 inhibitors. LoVo xenograft tumor-bearing mice were treated with 60 mpk MK-1775 BID for 2 days, 60 mpk MK-8776 BID for 2 days, or the combination of MK-1775 and MK-8776 each at 60 mpk BID for 2 days. Tumors were collected at 2, 24, and 48 hours following the final dose. A, LoVo tumor lysates were analyzed by Western blot for pCHK1S345. B, Tumor sections were fixed and analyzed by immunohistochemistry (IHC). Representative images for gamma H2AX at 2 hours and 48 hours post final dose are shown. C, Quantitative analysis of IHC for both phospho-CHK1S345 and gamma H2AX (n=3); one-way ANOVA analyses *P<0.05. **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23148684), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CDC2/CDK1 by Western Blot

Detection of Human CDC2/CDK1 by Western Blot

DNA damage response incurred by MK-1775 and MK-8776 is dependent on CDK activity.A, Resistant (H460) or sensitive (LoVo) cells were treated with concentrations of MK-1775 and MK-8776 described for Figure \n4, or 1 uM nocodazole for control. After 24 hours, cells were harvested and lysates analyzed by Western blot for caspase-dependent cleaved PARP (PARP*). B, A2058, HT-29, and LoVo cells were treated for 30 minutes with either DMSO or the indicated concentration of CDK inhibitor (SCH-727965). Following this pretreatment, further DMSO or concentrations of MK-1775 and MK-8776 used in Figures \n3 and\n4 (125 nM MK-1775 plus 150 nM MK-8776 in A2058; 125 nM MK-1775 plus 300 nM MK-8776 in HT-29, and 40 nM MK-1775 plus 75 nM MK-8776 in LoVo) were added to the cells for an additional 2 hours before cells were harvested and lysates analyzed by Western blot for phosphorylated CHK1S345, indicative of activated DNA damage response. C, LoVo cells were treated for 2 hours with 75 nM MK-1775 alone or in combination with 150 nM MK-8776, as indicated. Cells were harvested and lysates analyzed by Western blot for the proteins and phosphoproteins indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23148684), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CDC2/CDK1 by Western Blot

Detection of Human CDC2/CDK1 by Western Blot

DNA damage response incurred by MK-1775 and MK-8776 is dependent on CDK activity.A, Resistant (H460) or sensitive (LoVo) cells were treated with concentrations of MK-1775 and MK-8776 described for Figure \n4, or 1 uM nocodazole for control. After 24 hours, cells were harvested and lysates analyzed by Western blot for caspase-dependent cleaved PARP (PARP*). B, A2058, HT-29, and LoVo cells were treated for 30 minutes with either DMSO or the indicated concentration of CDK inhibitor (SCH-727965). Following this pretreatment, further DMSO or concentrations of MK-1775 and MK-8776 used in Figures \n3 and\n4 (125 nM MK-1775 plus 150 nM MK-8776 in A2058; 125 nM MK-1775 plus 300 nM MK-8776 in HT-29, and 40 nM MK-1775 plus 75 nM MK-8776 in LoVo) were added to the cells for an additional 2 hours before cells were harvested and lysates analyzed by Western blot for phosphorylated CHK1S345, indicative of activated DNA damage response. C, LoVo cells were treated for 2 hours with 75 nM MK-1775 alone or in combination with 150 nM MK-8776, as indicated. Cells were harvested and lysates analyzed by Western blot for the proteins and phosphoproteins indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23148684), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Western Blot

0.2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 4 reviews rated 3.8 using AF888 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDC2/CDK1

CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent Kinase 1), is a member of the CDK family of serine/threonine kinases. The CDKs are important regulators of cell cycle progression and their activities are largely controlled by association with Cyclins, and activating and inhibitory phosphorylations. Entry into mitosis is initiated by CDC2. Full activation of CDC2 requires phosphorylation at T161 and association with CyclinB. In contrast, phosphorylation of CDC2 at Y15 and T14 during the G2-phase of the cell cycle inhibits activity, and dephosphorylation of Y15 and T14 by CDC25 phosphatase during late G2 restores activity.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Entrez Gene IDs

983 (Human); 12534 (Mouse); 54237 (Rat)

Gene Symbol

CDK1

Additional CDC2/CDK1 Products

Product Documents for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

For research use only

Related Research Areas

Cell Cycle
Intracellular Kinases

Citations for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

Customer Reviews for Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody (4)

3.8 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
75%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody AF888
  • Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: PC-12 rat adrenal pheochromocytoma cell line
    Species: Rat
    Verified Customer | Posted 01/25/2018
    Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody AF888
  • Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: PANC-1 human pancreatic carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/17/2018
    Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody AF888

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