Human/Mouse/Rat Phospho-CDC2/CDK1 (Y15) Antibody

(2 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat CDC2 when phosphorylated at Y15.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing CDC2 Y15 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed MCF-7 human breast cancer cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Human Phospho-CDC2 (Y15) by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 0.2 μg/mL nocodazole or 12 μM aphidicolin or 1 mM hydroxurea for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDC2/CDK1
CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent Kinase 1), is a member of the CDK family of serine/threonine kinases. The CDKs are important regulators of cell cycle progression and their activities are largely controlled by association with Cyclins, and activating and inhibitory phosphorylations. Entry into mitosis is initiated by CDC2. Full activation of CDC2 requires phosphorylation at T161 and association with CyclinB. In contrast, phosphorylation of CDC2 at Y15 and T14 during the G2-phase of the cell cycle inhibits activity, and dephosphorylation of Y15 and T14 by CDC25 phosphatase during late G2 restores activity.
  • Long Name:
    Cell Division Cycle 2/Cyclin-dependent Kinase 1
  • Entrez Gene IDs:
    983 (Human); 12534 (Mouse); 54237 (Rat)
  • Alternate Names:
    CDC2; CDC28A; CDC2MGC111195; CDK1; cell cycle controller CDC2; Cell division control protein 2 homolog; Cell division protein kinase 1; cyclin-dependent kinase 1; DKFZp686L20222; EC 2.7.11.22; EC 2.7.11.23; G1 to S and G2 to M; p34 protein kinase; P34CDC2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Unique functions of CHK1 and WEE1 underlie synergistic anti-tumor activity upon pharmacologic inhibition.
    Authors: Guertin, Amy D, Martin, Melissa, Roberts, Brian, Hurd, Melissa, Qu, Xianlu, Miselis, Nathan R, Liu, Yaping, Li, Jing, Feldman, Igor, Benita, Yair, Bloecher, Andrew, Toniatti, Carlo, Shumway, Stuart D
    Cancer Cell Int, 2012;12(1):45.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. The p75 neurotrophin receptor interacts with multiple MAGE proteins.
    Authors: Tcherpakov M, Bronfman FC, Conticello SG, Vaskovsky A, Levy Z, Niinobe M, Yoshikawa K, Arenas E, Fainzilber M
    J. Biol. Chem., 2002;277(51):49101-4.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
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