Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antibody

Detection of Human Phospho-RSK (S380) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA in the presence or absence of the indicated concentrations of the MEK1/2 inhibitor U0126 for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK (S380) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-RSK (S380) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20, loaded at 0.2 mg/mL. A specific band was detected for Phospho-RSK (S380) at approximately 90 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Phospho-RSK (S380) Pan Specific in Mouse Embryo. RSK phosphorylated at S380 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to retinal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Phospho-RSK (S380) Pan Specific in Mouse Embryo. RSK phosphorylated at S380 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-RSK (S380) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF889) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to mesenchyme. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.