PP2A Catalytic Subunit Antibody (234529)

R&D Systems | Catalog # MAB1653

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 234529
View all PP2A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2A-alpha catalytic subunit
Trp209-Leu309
Accession # P67775

Specificity

Detects endogenous human, mouse, and rat PP2A in Western blots. Because PP2A-alpha and PP2A-beta catalytic subunits are identical in the region used for the immunogen, it is expected that both isoforms will be detected by the antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for PP2A Catalytic Subunit Antibody (234529)

Detection of Human and Mouse PP2A Catalytic Subunit, antibody by Western Blot.

Detection of Human and Mouse PP2A Catalytic Subunit, by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP2A Catalytic Subunit, Monoclonal Antibody (Catalog # MAB1653) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PP2A Catalytic Subunit, at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PP2A Catalytic Subunit Antibody (234529)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line

Reviewed Applications

Read 4 reviews rated 4.3 using MAB1653 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2A

Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.

Alternate Names

PP2CA, PPP2CA, RP-C

Entrez Gene IDs

5515 (Human); 51792 (Mouse)

Gene Symbol

PPP2CA

UniProt

P67775

Additional PP2A Products

Product Documents for PP2A Catalytic Subunit Antibody (234529)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PP2A Catalytic Subunit Antibody (234529)

For research use only

Customer Reviews for PP2A Catalytic Subunit Antibody (234529) (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat PP2A Catalytic Subunit Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    PP2A Catalytic Subunit Antibody (234529) MAB1653
  • Human/Mouse/Rat PP2A Catalytic Subunit Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human/Mouse/Rat PP2A Catalytic Subunit Antibody
    Name: Anonymous
    Application: Luminex
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/08/2018
  • Human/Mouse/Rat PP2A Catalytic Subunit Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma and Serum
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail