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Human/Mouse/Rat Total PP2A DuoSet IC ELISA

Catalog #: DYC1653-2
3 Citations Datasheet / COA / SDS

Discontinued Product

DYC1653-2 has been discontinued.
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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human/Mouse/Rat Total PP2A DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
625.0 - 20,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PP2A

Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits; a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab). PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.

Entrez Gene IDs:
5515 (Human); 51792 (Mouse)
Alternate Names:
EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Citations for Human/Mouse/Rat Total PP2A DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Protein phosphatase 2A (PP2A) regulates low density lipoprotein uptake through regulating sterol response element-binding protein-2 (SREBP-2) DNA binding.
    Authors: Rice L, Donigan M, Yang M, Liu W, Pandya D, Joseph B, Sodi V, Gearhart T, Yip J, Bouchard M, Nickels J
    J Biol Chem, 2014-04-26;289(24):17268-79.
    Species: Human
    Sample Types: Cell Lysates
  2. Folate is related to phosphorylated neurofilament-H and P-tau (Ser396) in rat brain.
    Authors: Obeid R, Schlundt J, Umanskaya N, Herrmann W, Herrmann M
    J. Neurochem., 2011-05-13;117(6):1047-54.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Carbon dioxide inhalation causes pulmonary inflammation.
    Authors: Abolhassani M, Guais A, Chaumet-Riffaud P, Sasco AJ, Schwartz L
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-01-09;296(4):L657-65.
    Species: Human
    Sample Types: Cell Lysates

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