|Detection of Human, Mouse, and Rat UBE2K/E2‑25K by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, U2OS human osteosarcoma cell line, BaF3 mouse pro-B cell line, Neuro‑2A mouse neuroblastoma cell line, and Y3‑Ag rat myeloid cell line. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat UBE2K/E2‑25K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6609) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human UBE2K/E2‑25K (5 ng/lane) was included. A specific band was detected for UBE2K/E2‑25K at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
UBE2K (Ubiquitin-conjugating enzyme E2; also HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length and it contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence.
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