Detection of Human, Mouse, and Rat UBE2K/E2‑25K by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, U2OS human osteosarcoma cell line, BaF3 mouse pro-B cell line, Neuro‑2A mouse neuroblastoma cell line, and Y3‑Ag rat myeloid cell line. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat UBE2K/E2‑25K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6609) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human UBE2K/E2‑25K (5 ng/lane) was included. A specific band was detected for UBE2K/E2‑25K at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
UBE2K (Ubiquitin-conjugating enzyme E2; also HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length and it contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence.
Have you used Human/Mouse/Rat UBE2K/E2-25K Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.