Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

R&D Systems | Catalog # IC6006A

R&D Systems
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 600380
View all ROR gamma t/RORC2/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

Human ROR gamma t peptide
Met1-Arg10
Accession # P51449

Specificity

Detects human and mouse ROR gamma t/RORC2 in direct ELISAs. In direct ELISAs, no cross-reactivity with human and mouse RORC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

Detection of ROR?t/RORC2/NR1F3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC6006A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of ROR?t/RORC2/NR1F3 antibody in Mouse CD4+/CD8+Thymocytes antibody by Flow Cytometry.

Detection of ROR gamma t/RORC2/NR1F3 in Mouse CD4+/CD8+Thymocytes by Flow Cytometry.

Mouse CD4+/CD8+thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC6006A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) and mouse CD4+/CD8+ thymocytes were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name

Retinoic Acid-Related Orphan Receptor gamma t

Alternate Names

RORC2

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449

Additional ROR gamma t/RORC2/NR1F3 Products

Product Documents for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

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Product Specific Notices for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

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