Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody

Catalog #: IC6006C
3 Citations Datasheet / COA / SDS

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IC6006C-100
IC6006C-025

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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry.

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Citations (3)

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Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse ROR gamma t/RORC2 in direct ELISAs. In direct ELISAs, no cross-reactivity with human and mouse RORC.

Source

Monoclonal Mouse IgG_2B Clone # 600380

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human ROR gamma t peptide
Met1-Arg10
Accession # P51449

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ROR?t/RORC2/NR1F3 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of ROR?t/RORC2/NR1F3 antibody in Mouse Thymocytes antibody by Flow Cytometry.

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Detection of ROR gamma t/RORC2/NR1F3 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name

Retinoic Acid-Related Orphan Receptor gamma t

Entrez Gene IDs

6097 (Human)

Alternate Names

IMD42;NR1F3;Nuclear receptor ROR-gamma;RORG;RZRG;RZR-GAMMA;TOR; ROR gamma t; RORC2

Product Datasheets

Product Datasheet

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Citations for Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Histone demethylase JARID1C/KDM5C regulates Th17 cells by increasing IL-6 expression in diabetic plasmacytoid dendritic cells
Authors: Audu, CO;Wolf, SJ;Joshi, AD;Moon, JY;Melvin, WJ;Sharma, SB;Davis, FM;Obi, AT;Wasikowski, R;Tsoi, LC;Barrett, EC;Mangum, KD;Bauer, TM;Kunkel, SL;Moore, BB;Gallagher, KA;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
T‑regulatory cells from patients with rheumatoid arthritis retain suppressor functions in vitro
Authors: Daniil Shevyrev, Valeriy Tereshchenko, Vladimir Kozlov, Alexey Sizikov, Oksana Chumasova, Veroniсa Koksharova
Experimental and Therapeutic Medicine
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Age-related changes in the immunomodulatory effects of human dental pulp derived mesenchymal stem cells on the CD4+ T cell subsets
Authors: RB Özgül Özde, AT Özdemir, C K?rmaz, A Eker Sar?b, E Karaöz, G Erman, HS Vatansever, N Mete Gökme
Cytokine, 2020-11-19;0(0):155367.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry