hh Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody IC6006C-100: R&D Systems

Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse ROR gamma t/RORC2 in direct ELISAs. In direct ELISAs, no cross-reactivity with human and mouse RORC.
  • Source
    Monoclonal Mouse IgG2B Clone # 600380
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human ROR gamma t peptide
    Met1-Arg10
    Accession # P51449
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of ROR gamma t/RORC2/NR1F3 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP‑conjugated Monoclonal Antibody (Catalog # IC6006C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.
  • Long Name:
    Retinoic Acid-Related Orphan Receptor gamma t
  • Entrez Gene IDs:
    6097 (Human)
  • Alternate Names:
    ROR gamma t; RORC2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PerCP-conjugated Isotype Control

Ctrl IC0041C
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information