Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody
Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody Summary
Met1-Arg10
Accession # P51449
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of ROR gamma t/RORC2/NR1F3 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 PerCP-conjugated Monoclonal Antibody (Catalog # IC6006C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.
Product Datasheets
Citation for Human/Mouse ROR gamma t/RORC2/NR1F3 PerCP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Age-related changes in the immunomodulatory effects of human dental pulp derived mesenchymal stem cells on the CD4+ T cell subsets
Authors: RB Özgül Özde, AT Özdemir, C K?rmaz, A Eker Sar?b, E Karaöz, G Erman, HS Vatansever, N Mete Gökme
Cytokine, 2020;0(0):155367.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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