Human/Mouse S100A6 Alexa Fluor® 532-conjugated Antibody

Catalog # Availability Size / Price Qty
AF4584X-100UG

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Human/Mouse S100A6 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse S100A6 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse S100A1 and recombinant mouse S100A4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse S100A6
Met1-Lys89
Accession # P14069
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Knockout Validated
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: S100A6

Mouse S100A6 (also prolactin receptor-associated protein and calcyclin) is a 10 kDa member of the S100 family of calcium-binding proteins. S100A6 is 89 amino acids (aa) in length and contains two calcium-binding EF-hand domains (aa 12‑47 and 48‑83). Intracellularly, S100A6 will both noncovalently homodimerize and heterodimerize with S100B plus SGT1. Extracellularly, it is secreted via a noncanonical pathway and binds to RAGE, inducing apoptosis. It is expressed by neurons, endothelium, fibroblasts and glandular epithelia. Mouse S100A6 is 99% and 96% aa identical to rat and human S100A6, respectively.

Long Name
S100 Calcium Binding Protein A6
Entrez Gene IDs
6277 (Human); 20200 (Mouse); 85247 (Rat)
Alternate Names
2A9; CABP; CACY; CACY5B10; calcyclin; Growth factor-inducible protein 2A9; MLN 4; PRA; PRAS100 calcium binding protein A6 (calcyclin); Prolactin receptor-associated protein; protein S100-A6; S100 calcium binding protein A6; S100 calcium-binding protein A6 (calcyclin); S100 calcium-binding protein A6; S100A6

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