Mouse S100A6 (also prolactin receptor-associated protein and calcyclin) is a 10 kDa member of the S100 family of calcium-binding proteins. S100A6 is 89 amino acids (aa) in length and contains two calcium-binding EF-hand domains (aa 12‑47 and 48‑83). Intracellularly, S100A6 will both noncovalently homodimerize and heterodimerize with S100B plus SGT1. Extracellularly, it is secreted via a noncanonical pathway and binds to RAGE, inducing apoptosis. It is expressed by neurons, endothelium, fibroblasts and glandular epithelia. Mouse S100A6 is 99% and 96% aa identical to rat and human S100A6, respectively.
Human/Mouse S100A6 Biotinylated Antibody
R&D Systems | Catalog # BAF4584
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse S100A6
Met1-Lys89
Accession # P14069
Met1-Lys89
Accession # P14069
Specificity
Detects human and mouse S100A6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) S100A1, rhS100A2, and recombinant mouse S100A4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse S100A6 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human and mouse S100A6
Sample: Recombinant Human and mouse S100A6
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: S100A6
Long Name
S100 Calcium Binding Protein A6
Alternate Names
CACY, MLN 4, PRA
Gene Symbol
S100A6
UniProt
Additional S100A6 Products
Product Documents for Human/Mouse S100A6 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse S100A6 Biotinylated Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars