Human/Mouse SorCS3 Antibody

Catalog # Availability Size / Price Qty
MAB3067
MAB3067-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human/Mouse SorCS3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SorCS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SorCS1 is observed.
Source
Monoclonal Rat IgG2A Clone # 339624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SorCS3
Ala131-Ser1122
Accession # Q8VI51
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SorCS3 (Catalog # 3326-SR)
Recombinant Mouse SorCS3 (Catalog # 3067-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorCS3

SorCS3 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein‑sorting 10 protein) family of receptors that includes sortilin, SorLA, and three SorCS proteins (1, 2). It is synthesized as an ~1220 amino acid (aa) preproform with a 33 aa signal sequence and a 100 aa propeptide. After proteolytic release of the propeptide at a furin‑type consensus sequence, the mature SorCS3 is a 100‑110 kDa transmembrane protein with a 992 aa extracellular/lumenal domain (ECD). Mouse and human SorCS3 ECD share 92% aa sequence identity. They also share ~70% and ~45% aa identity with SorCS1 and SorCS2 ECDs, respectively. The ECD contains an imperfect leucine‑rich repeat (LRR) and a Vps10p domain that binds both pro- and mature NGF (2, 3). The metalloproteinase TACE/ADAM17 is able to cleave SorCS3 near the membrane either constitutively, or at an increased rate when induced by phorbol esters (4). The shed ECD is able to bind PDGF‑BB and the NGF propeptide (4). Unlike sortilin, the SorCS3 propeptide has no known function; it does not block NGF binding or propeptide cleavage (3, 5). SorCS3 is predominantly expressed on the plasma membrane (3). It can be slowly internalized but, despite the presence of a sorting domain, there is no evidence for SorCS3‑mediated intracellular trafficking activity (3). It is expressed in the embryonic and adult central nervous system in areas distinct from that of SorCS1 and SorCS2 (1). Neuronal activity upregulates SorCS3 expression in the hippocampus (1).

References
  1. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
  2. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  3. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.
  4. Hermey, G. et al. (2006) Biochem. J. 395:285.
  5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
Long Name
Sortilin-related VPS10 Domain Containing Receptor 3
Entrez Gene IDs
22986 (Human); 66673 (Mouse); 294043 (Rat)
Alternate Names
6330404A12Rik; KIAA1059SORCS; SorCS3; sortilin-related VPS10 domain containing receptor 3; VPS10 domain receptor protein SORCS 3 (SORCS3); VPS10 domain-containing receptor SorCS3

Product Datasheets

Citations for Human/Mouse SorCS3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. SORCS1 and SORCS3 control energy balance and orexigenic peptide production
    Authors: A Subkhangul, AR Malik, G Hermey, O Popp, G Dittmar, T Rathjen, MN Poy, A Stumpf, PS Beed, D Schmitz, T Breiderhof, TE Willnow
    EMBO Rep., 2018;19(4):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017;13(6):e1006470.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse SorCS3 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse SorCS3 Antibody and earn rewards!

Have you used Human/Mouse SorCS3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review