Human/Mouse SorCS3 Antibody

Catalog # Availability Size / Price Qty
MAB3067
MAB3067-SP

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Detection of Chinese hamster SorCS3 by Western Blot
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Product Details
Citations (2)
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Human/Mouse SorCS3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SorCS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SorCS1 is observed.
Source
Monoclonal Rat IgG2A Clone # 339624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SorCS3
Ala131-Ser1122
Accession # Q8VI51
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SorCS3 (Catalog # 3326-SR)
Recombinant Mouse SorCS3 (Catalog # 3067-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Chinese hamster SorCS3 by Western Blot View Larger

Detection of Chinese hamster SorCS3 by Western Blot Altered subcellular localization of TrkB in primary neurons and brains from S1/3 KO miceAWorkflow of the cell surface proteome analysis in primary neurons.BResults of quantitative label‐free proteomics comparing the surface proteomes of WT and S1/3 KO primary cortical neurons. Plot represents –log10(P‐value) and log2 (relative levels S1/3KO/WT) obtained for each protein. For proteins that were detected in either S1/3 KO or WT samples only, the log2(S1/3 KO/WT) was set to 10 or ‐10, respectively. Threshold values are 1.3 for –log10(P‐value) and ±0.3 for log2(S1/3 KO/WT). Proteins showing higher (green) or lower (red) surface levels in S1/3 KO as compared to WT neurons are indicated. Proteins with unchanged cell surface levels are indicated in blue. n = 3 biological replicates/group (with each biological replicate run in two technical replicates).CScheme of brain membrane fractionation used to analyze subcellular localization of TrkB in vivo (see method section for details).DRepresentative Western blotting of TrkB in various brain membrane fractions obtained as described in panel (C). Highlighted lanes represent P3 and P2 fractions, quantified in (E). Detection of PSD95, synaptophysin, and Golgin 97 was used to assess the accuracy of subcellular fractionation.EQuantification of total TrkB distribution between P3 (light membrane fraction) and P2 (synaptosomal fraction) from Western blots exemplified in panel (D) (n = 5 mice/group, 7–12 weeks of age). The TrkB pool is shifted toward the light membrane (P3) fraction in S1/3 KO brains. Data are shown as mean ± SEM and analyzed using two‐way ANOVA with Bonferroni post‐test. ****P < 0.0001.F, GCo‐immunoprecipitation of SORCS1 and SORCS3 with TrkB. TrkB was immunoprecipitated from transiently transfected Chinese hamster ovary cells and the presence of SORCS1 or SORCS3 in the immunoprecipitates documented by Western blotting. Panel input shows levels of the indicated proteins in the cell lysates prior to immunoprecipitation. Panel IP documents co‐immunoprecipitation of SORCS1 (F) and SORCS3 (G) from cells expressing (lanes 3) but not from cells lacking TrkB (lanes 2). The experiment was replicated twice.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29440124), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorCS3

SorCS3 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein‑sorting 10 protein) family of receptors that includes sortilin, SorLA, and three SorCS proteins (1, 2). It is synthesized as an ~1220 amino acid (aa) preproform with a 33 aa signal sequence and a 100 aa propeptide. After proteolytic release of the propeptide at a furin‑type consensus sequence, the mature SorCS3 is a 100‑110 kDa transmembrane protein with a 992 aa extracellular/lumenal domain (ECD). Mouse and human SorCS3 ECD share 92% aa sequence identity. They also share ~70% and ~45% aa identity with SorCS1 and SorCS2 ECDs, respectively. The ECD contains an imperfect leucine‑rich repeat (LRR) and a Vps10p domain that binds both pro- and mature NGF (2, 3). The metalloproteinase TACE/ADAM17 is able to cleave SorCS3 near the membrane either constitutively, or at an increased rate when induced by phorbol esters (4). The shed ECD is able to bind PDGF‑BB and the NGF propeptide (4). Unlike sortilin, the SorCS3 propeptide has no known function; it does not block NGF binding or propeptide cleavage (3, 5). SorCS3 is predominantly expressed on the plasma membrane (3). It can be slowly internalized but, despite the presence of a sorting domain, there is no evidence for SorCS3‑mediated intracellular trafficking activity (3). It is expressed in the embryonic and adult central nervous system in areas distinct from that of SorCS1 and SorCS2 (1). Neuronal activity upregulates SorCS3 expression in the hippocampus (1).

References
  1. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
  2. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  3. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.
  4. Hermey, G. et al. (2006) Biochem. J. 395:285.
  5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
Long Name
Sortilin-related VPS10 Domain Containing Receptor 3
Entrez Gene IDs
22986 (Human); 66673 (Mouse); 294043 (Rat)
Alternate Names
6330404A12Rik; KIAA1059SORCS; SorCS3; sortilin-related VPS10 domain containing receptor 3; VPS10 domain receptor protein SORCS 3 (SORCS3); VPS10 domain-containing receptor SorCS3

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Citations for Human/Mouse SorCS3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SORCS1 and SORCS3 control energy balance and orexigenic peptide production
    Authors: A Subkhangul, AR Malik, G Hermey, O Popp, G Dittmar, T Rathjen, MN Poy, A Stumpf, PS Beed, D Schmitz, T Breiderhof, TE Willnow
    EMBO Rep., 2018-02-12;19(4):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Prions amplify through degradation of the VPS10P sorting receptor sortilin
    Authors: K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi
    PLoS Pathog., 2017-06-30;13(6):e1006470.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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