Human/Mouse UCP1 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6158T-100UG
R&D Systems Antibodies
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Human/Mouse UCP1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse UCP1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 536435
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
3T3‑L1 mouse embryonic fibroblast adipose-like cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: UCP1

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Long Name
Uncoupling Protein 1
Entrez Gene IDs
7350 (Human); 22227 (Mouse); 24860 (Rat)
Alternate Names
SLC25A7; SLC25A7mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; UCP 1; UCP; UCP1; uncoupling protein 1 (mitochondrial, proton carrier)

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