Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Xenograft

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 536435
View all UCP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874

Specificity

Detects human and mouse UCP1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse UCP1 PE‑conjugated Antibody

Detection of UCP1 antibody in Human Preadipocytes antibody by Flow Cytometry.

Detection of UCP1 in Human Preadipocytes by Flow Cytometry.

Human preadipocytes were stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of UCP1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry.

Detection of UCP1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 PE-conjugated Monoclonal Antibody (Catalog # IC6158P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse UCP1 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human preadipocytes and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: UCP1

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Long Name

Uncoupling Protein 1

Alternate Names

SLC25A7, Thermogenin

Entrez Gene IDs

7350 (Human); 22227 (Mouse); 24860 (Rat)

Gene Symbol

UCP1

UniProt

P25874 (Human)

Additional UCP1 Products

Product Documents for Human/Mouse UCP1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse UCP1 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse UCP1 PE‑conjugated Antibody

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