Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 536435
View all UCP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human UCP1
Met1-Thr307
Accession # P25874

Specificity

Detects human and mouse UCP1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse UCP1 Antibody

Detection of Human UCP1 antibody by Western Blot.

Detection of Human UCP1 by Western Blot.

Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Mouse UCP1 antibody by Western Blot.

Detection of Mouse UCP1 by Western Blot.

Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCP1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
UCP1 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

UCP1 in Human Mesenchymal Stem Cells.

UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of UCP1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry.

Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Mouse UCP1 antibody by Simple WesternTM.

Detection of Mouse UCP1 by Simple WesternTM.

Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse UCP1 by Immunohistochemistry

Detection of Mouse UCP1 by Immunohistochemistry

IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA levels of Ucp1 and Irx3 in IWAT (B) and BAT (C) from mice subjected to PBS or CL-316,243 for 10 days. For A–C, gene expression was normalized to 36b4. (D–E) Protein levels of Ucp1 and Irx3 (left) and the quantification value of Irx3 relative to Hsp90 (right) in IWAT (D) and BAT (E) in the mice subjected to PBS or CL-316,243 treatment. (F) Representative images of immunohistochemical staining in IWAT from mice subjected to PBS or CL-316,243 treatment (scale bar, 50 μm for the upper and middle panels, and 100 μm for the bottom panel). (G) PET-CT, gross image, HE staining (scale bar, 20 μm), and electron microscopy (scale bar, 5 μm) of the browning WAT from pheochromocytoma patients. (H) Representative images of immunohistochemical staining in oWAT from pheochromocytoma patients (scale bar, 100 μm for the upper panel, and 50 μm for the bottom panel). Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. The results are representative of at least three independent experiments. IWAT, inguinal white adipose tissue. BAT, brown adipose tissue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28988979), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse UCP1 by Immunohistochemistry

Detection of Mouse UCP1 by Immunohistochemistry

IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA levels of Ucp1 and Irx3 in IWAT (B) and BAT (C) from mice subjected to PBS or CL-316,243 for 10 days. For A–C, gene expression was normalized to 36b4. (D–E) Protein levels of Ucp1 and Irx3 (left) and the quantification value of Irx3 relative to Hsp90 (right) in IWAT (D) and BAT (E) in the mice subjected to PBS or CL-316,243 treatment. (F) Representative images of immunohistochemical staining in IWAT from mice subjected to PBS or CL-316,243 treatment (scale bar, 50 μm for the upper and middle panels, and 100 μm for the bottom panel). (G) PET-CT, gross image, HE staining (scale bar, 20 μm), and electron microscopy (scale bar, 5 μm) of the browning WAT from pheochromocytoma patients. (H) Representative images of immunohistochemical staining in oWAT from pheochromocytoma patients (scale bar, 100 μm for the upper panel, and 50 μm for the bottom panel). Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. The results are representative of at least three independent experiments. IWAT, inguinal white adipose tissue. BAT, brown adipose tissue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28988979), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human UCP1 by Western Blot

Detection of Human UCP1 by Western Blot

BMP7 upregulated browning markers in both SC and DN derived differentiated adipocytes. SC and DN preadipocytes were differentiated and treated as in Figure 1. (A) Browning content as estimated by BATLAS Webtool (n = 9); (B) texture sum variance as quantified by laser-scanning cytometry (n = 5); quantification of UCP1 gene expression by (C) RNA-sequencing (n = 9) and (D) RT-qPCR normalized to GAPDH (n = 5). (E) UCP1 protein expression normalized to beta -actin (n = 5); (F) representative UCP1 immunostaining images visualized by confocal microscopy. Nucleus was stained using propidium iodide (PI), BF represents bright field image. Scale bars represent 10 μm. (G) Quantification of UCP1 immunostaining intensity assisted by laser-scanning cytometry (n = 4000 cells of 4 donors). Data expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Statistics: Paired t-test (A,B,G), GLM (C), and one-way ANOVA with Tukey’s post-test (D,E). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832860), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse UCP1 by Western Blot

Detection of Mouse UCP1 by Western Blot

MYPT1 is crucial for scWAT beiging in mice. b UCP1 immunoblotting of mitochondrial fraction of scWATs from AAV-injected Mypt1flox/flox mice (left). Densitometric quantification of UCP1 immunoblotting (right). n = 7 per group. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36175407), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse UCP1 by Western Blot

Detection of Mouse UCP1 by Western Blot

MYPT1 is crucial for scWAT beiging in mice. gMypt1+/flox::Pdgfra-Cre mice exposed to 12 °C for 1 week (upper left). Immunoblotting of mitochondrial fraction of scWATs (lower left). Densitometric quantification of UCP1 immunoblot (right). n = 5 per group. P-JMJD1A immunoblotting of scWAT (inset). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36175407), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human UCP1 by Western Blot

Detection of Human UCP1 by Western Blot

Expression of thermogenic markers was elevated following long-term rosiglitazone treatment and subsided upon beige (B) to white (W) transition. Human primary abdominal subcutaneous preadipocytes were differentiated to B for 14 days (B14), then either the B culture conditions were applied for an additional 14 days (B21 and B28) or were replaced by a W differentiation medium (B14W7 and B14W14). As a negative control, W adipocytes were differentiated by their specific cocktail (W14, W21, and W28). UCP1 (A) gene, (B) protein, (C) CIDEA, and (D) LEP gene expression. Gene expression was normalized to GAPDH and protein expression to beta -actin. Data are presented as mean ± SD. n = 6. * p < 0.05, ** p < 0.01. We carried out statistics using Friedman’s test with Dunn’s multiple comparison test (A) or one-way ANOVA with Tukey’s post hoc test (B–D). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35337160), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of UCP1 by Western Blot

Detection of UCP1 by Western Blot

BAT converts to white-like adipose tissue of rabbits following birth. (a) Workflow for validation of BAT whitening in rabbits. (b) MRI images of rabbits iBAT and dBAT at different ages. (c) Fat fraction of rabbit iBAT and dBAT. (d) Anatomical images of rabbit dBAT and iBAT at 14 days and 4 months of age. (e) H&E staining results of rabbit iBAT and ingWAT at different ages. Scale bars, 50 µm. (f) UCP1 protein levels of rabbit iBAT and ingWAT at different ages. (g) UCP1 staining of iBAT_D14, ingWAT_D1 and ingWAT_D14 of rabbits. Scale bars, 20 µm. Data represent mean ± SEM, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36000239), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of UCP1 by Western Blot

Detection of UCP1 by Western Blot

BAT converts to white-like adipose tissue of rabbits following birth. (a) Workflow for validation of BAT whitening in rabbits. (b) MRI images of rabbits iBAT and dBAT at different ages. (c) Fat fraction of rabbit iBAT and dBAT. (d) Anatomical images of rabbit dBAT and iBAT at 14 days and 4 months of age. (e) H&E staining results of rabbit iBAT and ingWAT at different ages. Scale bars, 50 µm. (f) UCP1 protein levels of rabbit iBAT and ingWAT at different ages. (g) UCP1 staining of iBAT_D14, ingWAT_D1 and ingWAT_D14 of rabbits. Scale bars, 20 µm. Data represent mean ± SEM, **P < 0.01, ***P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36000239), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse UCP1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into adipocytes

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Simple Western

2.5 µg/mL
Sample: Mouse brown adipose tissue

Western Blot

0.5 µg/mL
Sample: Mouse brown adipose tissue and mouse adipose tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB6158 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UCP1

Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

Long Name

Uncoupling Protein 1

Alternate Names

SLC25A7, Thermogenin

Entrez Gene IDs

7350 (Human); 22227 (Mouse); 24860 (Rat)

Gene Symbol

UCP1

UniProt

P25874 (Human)

Additional UCP1 Products

Product Documents for Human/Mouse UCP1 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse UCP1 Antibody

For research use only

Citations for Human/Mouse UCP1 Antibody

Customer Reviews for Human/Mouse UCP1 Antibody (2)

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  • Human/Mouse UCP1 Antibody
    Name: Matthew Emmett
    Application: Western Blot
    Sample Tested: Primary Brown Adipocytes, Primary brown adipocytes- cultured and Brown adipose tissue
    Species: Mouse
    Verified Customer | Posted 07/12/2017
    Ucp1 antibody utilized for western blot and tested against Ucp1 KO brown adipose tissue. Emmett, M. J. et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature 546, 544-548 (2017).
  • Human/Mouse UCP1 Antibody
    Name: Aaron Brown
    Application: Western Blot
    Sample Tested: Brown adipose tissue
    Species: Human
    Verified Customer | Posted 07/11/2017
    Human/Mouse UCP1 Antibody MAB6158

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