Human/Mouse Wnt-5a Antibody

Catalog # Availability Size / Price Qty
MAB645
MAB645-SP
Detection of Human and Mouse Wnt‑5a by Western Blot.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Wnt-5a Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Wnt-5a in direct ELISAs and human and mouse Wnt‑5a in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11, or 16 is observed.
Source
Monoclonal Rat IgG2A Clone # 442625
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO-derived recombinant mouse Wnt-5a
Gln38-Lys380
Accession # P22725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue
 
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse Wnt-5a antibody by Western Blot. View Larger

Detection of Human and Mouse Wnt‑5a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-5a at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Wnt-5a antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23‑24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1‑2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.

References
  1. Miller, J.R. (2002) Genome Biol. 3:3001.
  2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  4. Topol, L. et al. (2003) J. Cell Biol 162:899.
  5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
  6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name
Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs
7474 (Human); 22418 (Mouse)
Alternate Names
hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a

Product Datasheets

Citations for Human/Mouse Wnt-5a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
    Authors: M Asem, AM Young, C Oyama, AG Claure De, Y Liu, J Yang, TS Hilliard, J Johnson, EI Harper, I Guldner, S Zhang, TM Page-Maybe, WJ Kaliney, MS Stack
    Cancer Res., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
    Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
    Respir. Res, 2017;18(1):52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells
    Authors: TC Huang, PT Lee, MH Wu, CC Huang, CY Ko, YC Lee, DY Lin, YW Cheng, KH Lee
    PLoS ONE, 2017;12(8):e0181034.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
    J Exp Clin Cancer Res, 2016;35(1):144.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
    Authors: Fuster J, Zuriaga M, Ngo D, Farb M, Aprahamian T, Yamaguchi T, Gokce N, Walsh K
    Diabetes, 2015;64(4):1235-48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014;4(0):6315.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma.
    Authors: Yap L, Ahmad M, Zabidi M, Chu T, Chai S, Lee H, Lim P, Wei W, Dawson C, Teo S, Khoo A
    Int J Oncol, 2014;44(5):1774-80.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  9. Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression.
    Authors: Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T
    Mol Oncol, 2014;8(8):1365-78.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
    Authors: Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, Zhou X, Ye L
    J Biol Chem, 2014;289(30):21028-39.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
    Authors: Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
    J Invest Dermatol, 2014;134(8):2183-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
    Authors: Barbolina MV, Burkhalter RJ, Stack MS
    Biochem. J., 2011;437(1):1-12.
    Species: Human
    Sample Types: Ascites Fluid
    Applications: Western Blot
  13. Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells.
    Authors: Hasan M, Rassenti L, Widhopf G, Yu J, Kipps T
    Leukemia, 0;33(3):653-661.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human/Mouse Wnt-5a Antibody
By Anonymous on 12/09/2020
Application: ICC/IF Sample Tested: E11.5 mouse embryo fixed in 4% PFA Species: Mouse

Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed).
Concentration used - 8ug/mL.