hh Human/Mouse Wnt-5a Antibody MAB645: R&D Systems

Human/Mouse Wnt-5a Antibody

(9 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse Wnt-5a in direct ELISAs and human and mouse Wnt‑5a in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11, or 16 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 442625
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO-derived recombinant mouse Wnt-5a
    Gln38-Lys380
    Accession # P22725
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Wnt‑5a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse Wnt‑5a Monoclonal Antibody (Catalog # MAB645) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt‑5a at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Wnt‑5a in Mouse Embryo. Wnt‑5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt‑5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23‑24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1‑2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.

  • References:
    1. Miller, J.R. (2002) Genome Biol. 3:3001.
    2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
    3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
    4. Topol, L. et al. (2003) J. Cell Biol 162:899.
    5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
    6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 5a
  • Entrez Gene IDs:
    7474 (Human); 22418 (Mouse)
  • Alternate Names:
    hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
    Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
    Respir. Res, 2017;18(1):52.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  2. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
    J Exp Clin Cancer Res, 2016;35(1):144.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
    Authors: Fuster J, Zuriaga M, Ngo D, Farb M, Aprahamian T, Yamaguchi T, Gokce N, Walsh K
    Diabetes, 2015;64(4):1235-48.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma.
    Authors: Yap L, Ahmad M, Zabidi M, Chu T, Chai S, Lee H, Lim P, Wei W, Dawson C, Teo S, Khoo A
    Int J Oncol, 2014;44(5):1774-80.
    Species: Human
    Sample Type: Protein
    Application: WB
  5. Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression.
    Authors: Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T
    Mol Oncol, 2014;8(8):1365-78.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014;4(0):6315.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
    Authors: Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
    J Invest Dermatol, 2014;134(8):2183-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
    Authors: Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, Zhou X, Ye L
    J Biol Chem, 2014;289(30):21028-39.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  9. Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
    Authors: Barbolina MV, Burkhalter RJ, Stack MS
    Biochem. J., 2011;437(1):1-12.
    Species: Human
    Sample Type: Ascites Fluid
    Application: WB
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Rat IgG HRP-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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