Human/Mouse Wnt-5a Antibody
Human/Mouse Wnt-5a Antibody Summary
Gln38-Lys380
Accession # P22725
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Wnt‑5a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-5a at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-5a
Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23‑24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1‑2).
Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.
- Miller, J.R. (2002) Genome Biol. 3:3001.
- Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
- Veeman, M.T. et al. (2003) Developmental Cell 5:367.
- Topol, L. et al. (2003) J. Cell Biol 162:899.
- Torres, M. et al. (1996) J. Cell Biol. 133:1123.
- Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Product Datasheets
Citations for Human/Mouse Wnt-5a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Increased MARCKS Activity in BRAF Inhibitor-Resistant Melanoma Cells Is Essential for Their Enhanced Metastatic Behavior Independent of Elevated WNT5A and IL-6 Signaling
Authors: V Yadav, N Jobe, SR Satapathy, P Mohapatra, T Andersson
Cancers, 2022;14(24):.
Species: Human
Sample Types: Protein Extracts
Applications: Western Blot -
YAP-dependent Wnt5a induction in hypertrophic adipocytes restrains adiposity
Authors: GJ Lee, YJ Kim, B Park, S Yim, C Park, H Roh, Y Moon, JK Seong, H Park
Cell Death & Disease, 2022;13(4):407.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
Authors: T Radaszkiew, M Nosková, K Gömöryová, O Vondálová, KA Radaszkiew, M Picková, R Víchová, T Gybe?, K Kaiser, L Demková, L Ku?erová, T Bárta, D Pot?šil, Z Zdráhal, K Sou?ek, V Bryja
Elife, 2021;10(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
HDAC6 suppresses microRNA-199a transcription and augments HPV-positive cervical cancer progression through Wnt5a upregulation
Authors: Y Shao, F Zhu, S Zhu, L Bai
The international journal of biochemistry & cell biology, 2021;0(0):106000.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
Authors: M Asem, AM Young, C Oyama, AG Claure De, Y Liu, J Yang, TS Hilliard, J Johnson, EI Harper, I Guldner, S Zhang, TM Page-Maybe, WJ Kaliney, MS Stack
Cancer Res., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells
Authors: TC Huang, PT Lee, MH Wu, CC Huang, CY Ko, YC Lee, DY Lin, YW Cheng, KH Lee
PLoS ONE, 2017;12(8):e0181034.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
Respir. Res, 2017;18(1):52.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
Authors: Hoeke A Baarsma
J. Exp. Med, 2016;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
J Exp Clin Cancer Res, 2016;35(1):144.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
Authors: Fuster J, Zuriaga M, Ngo D, Farb M, Aprahamian T, Yamaguchi T, Gokce N, Walsh K
Diabetes, 2015;64(4):1235-48.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
Authors: Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, Zhou X, Ye L
J Biol Chem, 2014;289(30):21028-39.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression.
Authors: Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T
Mol Oncol, 2014;8(8):1365-78.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
Authors: Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
J Invest Dermatol, 2014;134(8):2183-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma.
Authors: Yap L, Ahmad M, Zabidi M, Chu T, Chai S, Lee H, Lim P, Wei W, Dawson C, Teo S, Khoo A
Int J Oncol, 2014;44(5):1774-80.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
Sci Rep, 2014;4(0):6315.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Diverse mechanisms for activation of Wnt signalling in the ovarian tumour microenvironment.
Authors: Barbolina MV, Burkhalter RJ, Stack MS
Biochem. J., 2011;437(1):1-12.
Species: Human
Sample Types: Ascites Fluid
Applications: Western Blot -
Wnt5a causes ROR1 to complex and activate cortactin to enhance migration of chronic lymphocytic leukemia cells.
Authors: Hasan M, Rassenti L, Widhopf G, Yu J, Kipps T
Leukemia, 0;33(3):653-661.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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Staining was done on E11.5 mouse embryo heart sections (Right Ventricle) (4% PFA fixed).
Concentration used - 8ug/mL.
