Human/Mouse Wnt-8b Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
AF3367T-100UG

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Human/Mouse Wnt-8b Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Wnt-8b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Wnt-8a is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt-8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-8b

Mouse Wnt-8b is a presumably 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 amino acids (aa) in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.

Long Name
Wingless-type MMTV Integration Site Family, Member 8b
Entrez Gene IDs
7479 (Human); 22423 (Mouse); 293990 (Rat)
Alternate Names
protein Wnt-8b; wingless-type MMTV integration site family, member 8B; Wnt8b; Wnt-8b

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