Human/Mouse Wnt-8b Antibody Summary
Accession # Q9WUD6
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Wnt‑8b by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Wnt‑8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3367) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑8b at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse Wnt-8b is a presumably 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 amino acids (aa) in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.
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