Mouse Wnt-8b is a presumably 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 amino acids (aa) in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.
Human/Mouse Wnt‑8b Antibody
R&D Systems | Catalog # AF3367
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Specificity
Detects human and mouse Wnt-8b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Wnt-8a is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Wnt‑8b Antibody
Detection of Human Wnt‑8b by Western Blot.
Western blot shows lysates of NCI-N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Wnt-8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3367) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-8b at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Applications for Human/Mouse Wnt‑8b Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NCI‑N87 human gastric carcinoma cell line
Sample: NCI‑N87 human gastric carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Wnt-8b
Long Name
Wingless-type MMTV Integration Site Family, Member 8b
Alternate Names
Wnt8b
Gene Symbol
WNT8B
UniProt
Additional Wnt-8b Products
Product Documents for Human/Mouse Wnt‑8b Antibody
Product Specific Notices for Human/Mouse Wnt‑8b Antibody
For research use only
Related Research Areas
Citations for Human/Mouse Wnt‑8b Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
