Human/Mouse Wnt-8b Antibody

Catalog # Availability Size / Price Qty
AF3367
AF3367-SP
Detection of Human Wnt‑8b by Western Blot.
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Human/Mouse Wnt-8b Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Wnt-8b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Wnt-8a is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt-8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Wnt‑8b by Western Blot. View Larger

Detection of Human Wnt‑8b by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Wnt‑8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3367) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑8b at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-8b

Mouse Wnt-8b is a presumably 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 amino acids (aa) in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.

Long Name
Wingless-type MMTV Integration Site Family, Member 8b
Entrez Gene IDs
7479 (Human); 22423 (Mouse); 293990 (Rat)
Alternate Names
protein Wnt-8b; wingless-type MMTV integration site family, member 8B; Wnt8b; Wnt-8b

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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