Human/Mouse Wnt-9b Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human Wnt-9b in Western blots. Detects recombinant mouse Wnt-9b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Wnt-2, -6, -7a, -7b, -9a, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b,
    -10a, -10b, -11, or -16 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 406612
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Wnt‑9b
    Ala46-Glu90 & Lys228-Tyr298
    Accession # O35468.2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse Wnt‑9b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line, T47D human breast cancer cell line, and ME-180 human cervical epithelial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Human/Mouse Wnt‑9b Monoclonal Antibody (Catalog # MAB3669) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human Wnt-9b (20 ng) was included. A specific band for native Wnt-9b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-9b

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

  • References:
    1. http://www.stanford.edu/group/nusselab/cgi-bin/wnt/
    2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
    3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
    4. Qian, J. et al. (2003) Genomics 81:34.
    5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
    6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
    7. Park, J-S. et al. (2007) Development 134:2533.
    8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
    9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.
     
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 9b
  • Entrez Gene IDs:
    7484 (Human); 22412 (Mouse); 303586 (Rat)
  • Alternate Names:
    Protein Wnt-14b; wingless-type MMTV integration site family, member 15; wingless-type MMTV integration site family, member 9B; Wnt-14b; WNT14Bprotein Wnt-9b; Wnt-15; WNT15Protein Wnt-15; Wnt9b; Wnt-9b
Related Research Areas
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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