Human MS4A1/CD20 APC‑conjugated Antibody

R&D Systems | Catalog # FAB4225A

R&D Systems
Discontinued Product
FAB4225A has been discontinued. An alternative/replacement product is available: FAB4225R. View all CD20 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 396444
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human MS4A1/CD20
Met1-Pro297
Accession # P11836

Specificity

Detects human MS4A1/CD20. Stains human MS4A1/CD20 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MS4A1/CD20 APC‑conjugated Antibody

Detection of MS4A1/CD20 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry.

Detection of MS4A1/CD20 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human MS4A1/CD20 APC-conjugated Monoclonal Antibody (Catalog # FAB4225A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Detection of MS4A1/CD20 antibody in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP antibody by Flow Cytometry.

Detection of MS4A1/CD20 in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP was stained with either (A) Mouse Anti-Human MS4A1/CD20 APC-conjugated Monoclonal Antibody (Catalog # FAB4225A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MS4A1/CD20 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanning protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

UniProt

P11836

Additional CD20 Products

Product Documents for Human MS4A1/CD20 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human MS4A1/CD20 APC‑conjugated Antibody

For research use only

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