Human CD20 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4225R-100UG
Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry.
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Human CD20 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MS4A1/CD20. Stains human MS4A1/CD20 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 396444
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human MS4A1/CD20
Met1-Pro297
Accession # P11836
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry View Larger

Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (FAB4869P) and either (A) Mouse Anti-Human MS4A1/CD20 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB4225R) or (B) Mouse IgG1Alexa Fluor 647 Isotype Control (IC002R). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry View Larger

Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human MS4A1/CD20 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB4225R) or (B) Mouse IgG1 Isotype Control (IC002R) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name
Cluster of Differentiation 20
Entrez Gene IDs
931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)
Alternate Names
B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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