Human MyoD Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB5966N Datasheet / COA / SDS

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FAB5966N-100UG

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Human MyoD Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MyoD in direct ELISAs.

Source

Monoclonal Mouse IgG2a Clone # 474515

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human MyoD
Glu77-Gly215
Accession # P15172

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MyoD

MyoD (myoblast determination protein 1), also called Myf-3 (myogenic factor 3) is an ~44 kDa nuclear protein in the MyoD family of muscle-specific bHLH transcription factors. MyoD family members heterodimerize with E proteins and cooperate with MEF2 family transcription factors to regulate expression of skeletal muscle-specific genes. MyoD is essential for skeletal muscle differentiation. Acetylation at lysines 99, 102 and 104 further regulates its activity. Human MyoD shares 96% amino acid identity with mouse and rat MyoD over the sequence used as the immunogen.

Long Name

Myoblast Determination Protein 1

Entrez Gene IDs

4654 (Human); 17927 (Mouse); 337868 (Rat)

Alternate Names

bHLHc1; bHLHc1BHLHC1; Class C basic helix-loop-helix protein 1; MYF3; MYF3Myf-3; MyoD; MYOD1; MYODmyoblast determination protein 1; myogenic differentiation 1; Myogenic factor 3myf-3; PUM