Human MyoD Antibody

Detection of MyoD in C2C12 Mouse Cell Line and Rd. MyoD was detected in fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative) using Mouse Anti-Human MyoD Monoclonal Antibody (Catalog # MAB5966) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of MyoD by Western Blot Cdkn1c is required for the proper formation of iBAT.(A) Photograph of E18.5 WT and Cdkn1c-/+ (KOMAT) fetuses with position of iBAT depot highlighted by dotted black line. (B) H&E staining of iBAT sections of E18.5 WT and KOMAT iBAT. (C) QPCR of Cdkn1c and the adipocyte regulators Rb1, PPAR gamma, C/EBP alpha and C/EBP beta, pan-adipocyte genes Fabp4, Cidec and Plin1, BAT-selective genes Ppargc1a, Cidea, Prdm16, Ucp1 and Elovl3, mitochondrial genes Cycs and Cox2, and the skeletal muscle-selective genes Myf5 and Myod1 in E18.5 KOMAT iBAT relative to WT (n = 4 per genotype). (D) Mitochondrial DNA content of iBAT from E18.5 KOMAT iBAT relative to WT (n = 6 per genotype). (E) Western blot analysis of UCP1 and beta -ACTIN in E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. (F) Western blot analysis of CDKN1C, PRDM16 and beta -ACTIN in E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. (G) Western blot analysis of MYOD and GAPDH in E18.5 E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. Data expressed as mean ± SEM, t test. * P <0.05; ** P <0.01; *** P <0.005. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26963625), licensed under a CC-BY license. Not internally tested by R&D Systems.