Human MyoD Antibody

Catalog # Availability Size / Price Qty

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

Product Details
Citations (1)
Supplemental Products

Human MyoD Antibody Summary

Species Reactivity
Detects human MyoD in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 474515
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human MyoD
Accession # P15172
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
Immersion fixed C2C12 mouse myoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyoD

MyoD (myoblast determination protein 1), also called Myf-3 (myogenic factor 3) is an ~44 kDa nuclear protein in the MyoD family of muscle-specific bHLH transcription factors. MyoD family members heterodimerize with E proteins and cooperate with MEF2 family transcription factors to regulate expression of skeletal muscle-specific genes. MyoD is essential for skeletal muscle differentiation. Acetylation at lysines 99, 102 and 104 further regulates its activity. Human MyoD shares 96% amino acid identity with mouse and rat MyoD over the sequence used as the immunogen.

Long Name
Myoblast Determination Protein 1
Entrez Gene IDs
4654 (Human); 17927 (Mouse); 337868 (Rat)
Alternate Names
bHLHc1; bHLHc1BHLHC1; Class C basic helix-loop-helix protein 1; MYF3; MYF3Myf-3; MyoD; MYOD1; MYODmyoblast determination protein 1; myogenic differentiation 1; Myogenic factor 3myf-3; PUM

Product Datasheets

You must select a language.


Citation for Human MyoD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activation of the Wnt/ β-catenin pathway by an inflammatory microenvironment affects the myogenic differentiation capacity of human laryngeal mucosa mesenchymal stromal cells
    Authors: R Yang, X Yang, S Liu, L Ming, Z Zhou, Y Liang, Y Zhao, F Zhou, ZH Deng, Y Jin
    Stem Cells Dev., 2018;0(0):.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MyoD Antibody

There are currently no reviews for this product. Be the first to review Human MyoD Antibody and earn rewards!

Have you used Human MyoD Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review