Human MyoD Antibody

Catalog # Availability Size / Price Qty
MAB5966
MAB5966-SP

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Product Details
Citations (1)
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Human MyoD Antibody Summary

Species Reactivity
Human
Specificity
Detects human MyoD in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 474515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MyoD
Glu77-Gly215
Accession # P15172
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
Immersion fixed C2C12 mouse myoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyoD

MyoD (myoblast determination protein 1), also called Myf-3 (myogenic factor 3) is an ~44 kDa nuclear protein in the MyoD family of muscle-specific bHLH transcription factors. MyoD family members heterodimerize with E proteins and cooperate with MEF2 family transcription factors to regulate expression of skeletal muscle-specific genes. MyoD is essential for skeletal muscle differentiation. Acetylation at lysines 99, 102 and 104 further regulates its activity. Human MyoD shares 96% amino acid identity with mouse and rat MyoD over the sequence used as the immunogen.

Long Name
Myoblast Determination Protein 1
Entrez Gene IDs
4654 (Human); 17927 (Mouse); 337868 (Rat)
Alternate Names
bHLHc1; bHLHc1BHLHC1; Class C basic helix-loop-helix protein 1; MYF3; MYF3Myf-3; MyoD; MYOD1; MYODmyoblast determination protein 1; myogenic differentiation 1; Myogenic factor 3myf-3; PUM

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Citation for Human MyoD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activation of the Wnt/ β-catenin pathway by an inflammatory microenvironment affects the myogenic differentiation capacity of human laryngeal mucosa mesenchymal stromal cells
    Authors: R Yang, X Yang, S Liu, L Ming, Z Zhou, Y Liang, Y Zhao, F Zhou, ZH Deng, Y Jin
    Stem Cells Dev., 2018;0(0):.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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