Intracellular Staining by Flow Cytometry
|Detection of Nanog in BG01V Human Cells by Flow Cytometry. Three-day Endoderm-differentiated BG01V human embryonic stem cells (upper image) or untreated BG01V human embryonic stem cells (lower image) were stained with Goat Anti-Human Nanog PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1997P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
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