hh Human Nanog Antibody AF1997: R&D Systems

Human Nanog Antibody

(68 citations)
(4 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Nanog
    Trp153-Val305
    Accession # ABZ92376
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Nanog by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Chromatin Immunoprecipitation (ChIP)
Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Immunocytochemistry
Nanog in BG01V Human Stem Cells. Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Nanog in BG01V Human Stem Cells-derived Embryoid Body. Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Nanog in ADLF1 and FAB2 Stem Cell Lines. Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nanog
Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).
  • References:
    1. Mitsui, K. et al. (2003) Cell 11:631.
    2. Chambers, I. et al. (2003) Cell 113:643.
    3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

  • Long Name:
    Nanog Homeobox
  • Entrez Gene IDs:
    79923 (Human); 71950 (Mouse)
  • Alternate Names:
    FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

68 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)
    Authors: E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés
    Stem Cell Res, 2017;21(0):19-22.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status
    Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda
    Stem Cells Int, 2017;2017(0):7906843.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Differentiation-Defective Human Induced Pluripotent Stem Cells Reveal�Strengths and Limitations of the Teratoma Assay and In�Vitro Pluripotency Assays
    Authors: MJ Bouma, M van Iterso, B Janssen, CL Mummery, DCF Salvatori, C Freund
    Stem Cell Reports, 2017;8(5):1340-1353.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh
  5. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation
    Authors: CA Thomas, L Tejwani, CA Trujillo, PD Negraes, RH Herai, P Mesci, A Macia, YJ Crow, AR Muotri
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells
    Authors: E Baljinnyam, S Venkatesh, R Gordan, S Mareedu, J Zhang, LH Xie, EI Azzam, CK Suzuki, D Fraidenrai
    Physiol Rep, 2017;5(15):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017;12(4):814-827.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration
    Authors: JS Saini, B Corneo, JD Miller, TR Kiehl, Q Wang, NC Boles, TA Blenkinsop, JH Stern, S Temple
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders
    Authors: C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells
    Stem Cell Reports, 2016;7(3):496-507.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  11. Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming
    Authors: C Eguizabal
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  12. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016;11(4):e0152993.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  13. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients
    Elife, 2016;5(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application
    Stem Cell Res, 2016;17(2):379-390.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC PFA fixed
  15. Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow
    FEBS Open Bio, 2016;6(11):1054-1066.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  16. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts
    Stem Cell Res, 2016;17(2):444-447.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: IHC Fresh
  17. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
    PLoS ONE, 2016;11(9):e0161229.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IHC Not Specified
  18. Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient
    Stem Cell Res, 2016;17(2):289-291.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Fresh
  19. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
    Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
    Nat. Med., 2016;22(12):1421-1427.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  20. beta-Catenin and NF-kappaB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer.
    Authors: Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, Chi L, Filion L, Figeys D, Wang L
    Cell Death Differ, 2015;22(2):298-310.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  21. V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
    Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A
    PLoS ONE, 2015;10(3):e0118499.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer.
    Authors: Francescangeli F, Contavalli P, De Angelis M, Baiocchi M, Gambara G, Pagliuca A, Fiorenzano A, Prezioso C, Boe A, Todaro M, Stassi G, Castro N, Watanabe K, Salomon D, De Maria R, Minchiotti G, Zeuner A
    Cell Death Differ, 2015;22(10):1700-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport.
    Authors: Freibaum B, Lu Y, Lopez-Gonzalez R, Kim N, Almeida S, Lee K, Badders N, Valentine M, Miller B, Wong P, Petrucelli L, Kim H, Gao F, Taylor J
    Nature, 2015;525(7567):129-33.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  24. Metabolic rescue in pluripotent cells from patients with mtDNA disease.
    Authors: Ma H, Folmes C, Wu J, Morey R, Mora-Castilla S, Ocampo A, Ma L, Poulton J, Wang X, Ahmed R, Kang E, Lee Y, Hayama T, Li Y, Van Dyken C, Gutierrez N, Tippner-Hedges R, Koski A, Mitalipov N, Amato P, Wolf D, Huang T, Terzic A, Laurent L, Izpisua Belmonte J, Mitalipov S
    Nature, 2015;524(7564):234-8.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  25. Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
    Authors: Liu L, Wu L, Wei X, Ling J
    Stem Cells Dev, 2015;24(8):962-72.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  26. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014;4(0):3779.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  27. A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.
    Authors: Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima K, King R, Maminishkis A, Marugan J, Sharma R, Shure M, Temple S, Miller S, Bharti K
    Stem Cells Transl Med, 2014;3(8):911-22.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  28. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014;23(18):2225-36.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  29. Epigenetic reprogramming induces the expansion of cord blood stem cells.
    Authors: Chaurasia P, Gajzer D, Schaniel C, D'Souza S, Hoffman R
    J Clin Invest, 2014;124(6):2378-95.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  30. Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
    Authors: Bershteyn, Marina, Hayashi, Yohei, Desachy, Guillaum, Hsiao, Edward C, Sami, Salma, Tsang, Kathryn, Weiss, Lauren A, Kriegstein, Arnold R, Yamanaka, Shinya, Wynshaw-Boris, Anthony
    Nature, 2014;507(7490):99-103.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  31. Sox2 promotes tamoxifen resistance in breast cancer cells.
    Authors: Piva M, Domenici G, Iriondo O, Rabano M, Simoes B, Comaills V, Barredo I, Lopez-Ruiz J, Zabalza I, Kypta R, Vivanco M
    EMBO Mol Med, 2014;6(1):66-79.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  32. Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome.
    Authors: Kobayashi, Junko, Yoshida, Masashi, Tarui, Suguru, Hirata, Masataka, Nagai, Yusuke, Kasahara, Shingo, Naruse, Keiji, Ito, Hiroshi, Sano, Shunji, Oh, Hidemasa
    PLoS ONE, 2014;9(7):e102796.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  33. Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line.
    Authors: Wu, Yi-Lin, Ma, Shi-Rong, Peng, Tao, Teng, Zeng-Hui, Liang, Xiang-Ya, Guo, Guo-Zhen, Zhang, Hai-Feng, Li, Kang-Chu
    Int J Mol Sci, 2014;15(8):14180-90.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  34. Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
    Authors: Piao Y, Hung S, Lim S, Wong R, Ko M
    Stem Cells Transl Med, 2014;3(0):787-791.
  35. Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells.
    Authors: Nathwani B, Miller C, Yang T, Solimano J, Liao J
    Stem Cells Dev, 2014;23(2):115-23.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  36. Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
    Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
    PLoS ONE, 2014;9(1):e84547.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  37. Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
    Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris
    PLoS ONE, 2014;9(12):e116114.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  38. Efficient generation of human iPSCs by a synthetic self-replicative RNA.
    Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S
    Cell Stem Cell, 2013;13(2):246-54.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  39. Differential L1 regulation in pluripotent stem cells of humans and apes.
    Authors: Marchetto M, Narvaiza I, Denli A, Benner C, Lazzarini T, Nathanson J, Paquola A, Desai K, Herai R, Weitzman M, Yeo G, Muotri A, Gage F
    Nature, 2013;503(7477):525-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  40. A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells.
    Authors: Zhang R, Mjoseng H, Hoeve M, Bauer N, Pells S, Besseling R, Velugotla S, Tourniaire G, Kishen R, Tsenkina Y, Armit C, Duffy C, Helfen M, Edenhofer F, de Sousa P, Bradley M
    Nat Commun, 2013;4(0):1335.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  41. Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
    Authors: Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P
    Stem Cells Dev, 2013;22(23):3141-55.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  42. Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome.
    Authors: Bellin, Milena, Casini, Simona, Davis, Richard, D'Aniello, Cristina, Haas, Jessica, Ward-van Oostwaard, Dorien, Tertoolen, Leon G J, Jung, Christia, Elliott, David A, Welling, Andrea, Laugwitz, Karl-Lud, Moretti, Alessand, Mummery, Christin
    EMBO J, 2013;32(24):3161-75.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  43. The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients.
    Authors: Fatima A, Kaifeng S, Dittmann S, Xu G, Gupta M, Linke M, Zechner U, Nguemo F, Milting H, Farr M, Hescheler J, Saric T
    PLoS ONE, 2013;8(12):e83005.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  44. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  45. Sox2 expression in breast tumours and activation in breast cancer stem cells.
    Authors: Leis O, Eguiara A, Lopez-Arribillaga E, Alberdi MJ, Hernandez-Garcia S, Elorriaga K, Pandiella A, Rezola R, Martin AG
    Oncogene, 2012;31(11):1354-65.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  46. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2012;21(8):1272-86.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  47. The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
    Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K
    Stem Cells Int, 2012;2012(0):875059.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  48. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  49. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011;29(4):609-17.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  50. A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
    Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
    Stem Cells, 2011;29(10):1517-27.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  51. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  52. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
    Authors: Chayosumrit M, Tuch B, Sidhu K
    Biomaterials, 2010;31(3):505-14.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  53. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
    Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL
    Nature, 2010;465(7299):808-12.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  54. Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach.
    Authors: Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J
    PLoS ONE, 2010;5(12):e14397.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  55. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  56. Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
    Authors: Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K
    FEBS Lett., 2009;583(6):1029-33.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  57. Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
    Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(24):9826-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  58. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  59. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
    J. Cell. Biochem., 2009;106(5):867-77.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  60. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
    Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
    Biotechnol. Bioeng., 2009;102(6):1636-44.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  61. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
    Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L
    Cell Stem Cell, 2008;2(6):602-12.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  62. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
    Cell, 2007;131(5):861-72.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  63. Pedigreed primate embryonic stem cells express homogeneous familial gene profiles.
    Authors: Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G
    Stem Cells, 2007;25(11):2695-704.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: ICC
  64. Isolation of a novel population of multipotent adult stem cells from human hair follicles.
    Authors: Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M, Xu X
    Am. J. Pathol., 2006;168(6):1879-88.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  65. Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells.
    Authors: Tsai MS, Hwang SM, Tsai YL, Cheng FC, Lee JL, Chang YJ
    Biol. Reprod., 2005;74(3):545-51.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  66. Monitoring differentiation of human embryonic stem cells using real-time PCR.
    Authors: Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, Kubista M, Sartipy P
    Stem Cells, 2005;23(10):1460-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  67. Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.
    Authors: Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M
    Stem Cells, 2005;23(8):1035-43.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 68 Citations
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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WB, ELISA R-401-C-ABS
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Average Rating: 3.8 (Based on 4 reviews)

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We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human Nanog Antibody AF1997
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 ICC/IF Human Anonymous 11/23/2017
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Immunocytochemistry/Immunofluorescence Human Nanog Antibody AF1997
Immunocytochemistry/Immunofluorescence: Human Nanog Antibody [AF1997]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryonic stem cells
SpeciesHuman
  Very Good
 WB Mouse Anonymous 07/05/2017
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Summary

ApplicationWestern Blot
Sample Tested7TD1 mouse hybridoma cell line
SpeciesMouse
  Very Good
 ICC/IF Human Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedstem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsWorks nicely in immunostainings 1:50.
  Very Good
 WB Human Anonymous 12/15/2016
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Summary

ApplicationWestern Blot
Sample Testedstem cells
SpeciesHuman

Other Experimental Details

Other Experimental DetailsWorks nicely in Western blotting 1:5000.

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