Human Nanog Antibody

Name: Human Nanog Antibody
Brand: R&D Systems
Rating: 4 (5 reviews)

(80 citations)

(5 Reviews)

Datasheet / CoA / SDS

Western Blot

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Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation.

Chromatin Immunoprecipitation (ChIP)

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Immunocytochemistry

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Nanog in BG01V Human Stem Cells-derived Embryoid Body.

Immunocytochemistry

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Nanog in ADLF1 and FAB2 Stem Cell Lines.

Immunocytochemistry

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Product Details

Citations (80)

FAQs

Supplemental Products

Reviews

Species Reactivity

Human
Specificity

Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

See below
Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 10⁶ cells

See below
Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Human Nanog by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Chromatin Immunoprecipitation (ChIP) click image to view larger	Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Immunocytochemistry click image to view larger	Nanog in BG01V Human Stem Cells. Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry click image to view larger	Nanog in BG01V Human Stem Cells-derived Embryoid Body. Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry click image to view larger	Nanog in ADLF1 and FAB2 Stem Cell Lines. Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References:
1. Mitsui, K. et al. (2003) Cell 11:631.
2. Chambers, I. et al. (2003) Cell 113:643.
3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name:

Nanog Homeobox

Entrez Gene IDs:

79923 (Human); 71950 (Mouse)

Alternate Names:

FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

80 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation Authors: PJ Yang, WX Yuan, J Liu, JY Li, B Tan, C Qiu, XL Zhu, C Qiu, DM Lai, LH Guo, LY Yu Acta Pharmacol. Sin., 2018;0(0):. Species: Human Sample Type: Whole Cells Application: ICC High-efficiency RNA-based reprogramming of human primary fibroblasts Authors: I Kogut, SM McCarthy, M Pavlova, DP Astling, X Chen, A Jakimenko, KL Jones, A Getahun, JC Cambier, AMG Pasmooij, MF Jonkman, DR Roop, G Bilousova Nat Commun, 2018;9(1):745. Species: Human Sample Type: Whole Cells Application: ICC Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo Authors: OM Russell, I Fruh, PK Rai, D Marcellin, T Doll, A Reeve, M Germain, J Bastien, KA Rygiel, R Cerino, AW Sailer, M Lako, RW Taylor, M Mueller, RN Lightowler, DM Turnbull, SB Helliwell Sci Rep, 2018;8(1):1799. Species: Mouse Sample Type: Whole Tissue Application: IHC Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) Authors: E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés Stem Cell Res, 2017;21(0):19-22. Species: Human Sample Type: Whole Cells Application: ICC Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders Authors: C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione Cell Stem Cell, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration Authors: JS Saini, B Corneo, JD Miller, TR Kiehl, Q Wang, NC Boles, TA Blenkinsop, JH Stern, S Temple Cell Stem Cell, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier Nat Protoc, 2017;12(4):814-827. Species: Human Sample Type: Whole Cells Application: Flow Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells Authors: E Baljinnyam, S Venkatesh, R Gordan, S Mareedu, J Zhang, LH Xie, EI Azzam, CK Suzuki, D Fraidenrai Physiol Rep, 2017;5(15):. Species: Human Sample Type: Whole Cells Application: ICC Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation Authors: CA Thomas, L Tejwani, CA Trujillo, PD Negraes, RH Herai, P Mesci, A Macia, YJ Crow, AR Muotri Cell Stem Cell, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC Differentiation-Defective Human Induced Pluripotent Stem Cells Revealï¿½Strengths and Limitations of the Teratoma Assay and Inï¿½Vitro Pluripotency Assays Authors: MJ Bouma, M van Iterso, B Janssen, CL Mummery, DCF Salvatori, C Freund Stem Cell Reports, 2017;8(5):1340-1353. Species: Human Sample Type: Whole Tissue Application: IHC Fresh HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu Oncotarget, 2017;8(17):28074-28092. Species: Human Sample Type: Cell Lysates Application: WB Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda Stem Cells Int, 2017;2017(0):7906843. Species: Human Sample Type: Whole Cells Application: ICC Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9 Authors: Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li Stem Cell Res, 2017;22(0):29-32. Species: Human Sample Type: Whole Cells Application: ICC Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation Authors: CW Chang, CC Chang, KC Hsia, SY Tsai Stem Cell Res, 2017;27(0):21-24. Species: Human Sample Type: Whole Cells Application: ICC A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu Circ. Res, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel Stem Cell Res, 2017;23(0):73-76. Species: Human Sample Type: Whole Cells Application: ICC Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing Authors: G Xu, D Guo, F Wu, N Abbas, K Lai, F Yuan, K You, Y Liu, Y Zhuang, Y Wu, Y Xu, Y Chen, F Yang, T Pan, YX Li Stem Cell Res, 2017;27(0):38-41. Species: Human Sample Type: Whole Cells Application: ICC Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu Cell Death Discov, 2017;3(0):16105. Species: Human Sample Type: Cell Lysates Application: WB TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation Authors: N Yahata, Y Matsumoto, M Omi, N Yamamoto, R Hata Sci Rep, 2017;7(1):15557. Species: Human Sample Type: Whole Cells Application: ICC Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS) Authors: B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya Stem Cell Res, 2017;0(0):. Species: Human Sample Type: Whole Cells Application: ICC Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines Authors: L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya Stem Cell Res, 2017;24(0):155-159. Species: Human Sample Type: Whole Cells Application: ICC Capturing the biology of disease severity in a PSC-based model of familial dysautonomia Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer Nat. Med., 2016;22(12):1421-1427. Species: Human Sample Type: Whole Cells Application: ICC Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming Authors: C Eguizabal Stem Cells, 2016;0(0):. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow FEBS Open Bio, 2016;6(11):1054-1066. Species: Human Sample Type: Whole Cells Application: ICC Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient Stem Cell Res, 2016;17(2):289-291. Species: Human Sample Type: Whole Cells Application: IHC - Fresh A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells PLoS ONE, 2016;11(9):e0161229. Species: Human Sample Type: Cell Culture Supernates Application: IHC Not Specified An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts Stem Cell Res, 2016;17(2):444-447. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Cells Application: IHC Fresh Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells Stem Cell Reports, 2016;7(3):496-507. Species: Human Sample Type: Whole Cells Application: IHC Fresh Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application Stem Cell Res, 2016;17(2):379-390. Species: Human Sample Type: Whole Cells Application: IHC PFA fixed A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients Elife, 2016;5(0):. Species: Human Sample Type: Whole Cells Application: ICC A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan PLoS ONE, 2016;11(4):e0152993. Species: Human Sample Type: Whole Cells Application: IHC Fresh beta-Catenin and NF-kappaB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer. Authors: Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, Chi L, Filion L, Figeys D, Wang L Cell Death Differ, 2015;22(2):298-310. Species: Human Sample Type: Cell Lysates Application: WB Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability. Authors: Liu L, Wu L, Wei X, Ling J Stem Cells Dev, 2015;24(8):962-72. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Metabolic rescue in pluripotent cells from patients with mtDNA disease. Authors: Ma H, Folmes C, Wu J, Morey R, Mora-Castilla S, Ocampo A, Ma L, Poulton J, Wang X, Ahmed R, Kang E, Lee Y, Hayama T, Li Y, Van Dyken C, Gutierrez N, Tippner-Hedges R, Koski A, Mitalipov N, Amato P, Wolf D, Huang T, Terzic A, Laurent L, Izpisua Belmonte J, Mitalipov S Nature, 2015;524(7564):234-8. Species: Human Sample Type: Whole Cells Application: IHC - Not specified GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport. Authors: Freibaum B, Lu Y, Lopez-Gonzalez R, Kim N, Almeida S, Lee K, Badders N, Valentine M, Miller B, Wong P, Petrucelli L, Kim H, Gao F, Taylor J Nature, 2015;525(7567):129-33. Species: Human Sample Type: Whole Cells Application: ICC Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer. Authors: Francescangeli F, Contavalli P, De Angelis M, Baiocchi M, Gambara G, Pagliuca A, Fiorenzano A, Prezioso C, Boe A, Todaro M, Stassi G, Castro N, Watanabe K, Salomon D, De Maria R, Minchiotti G, Zeuner A Cell Death Differ, 2015;22(10):1700-13. Species: Human Sample Type: Whole Cells Application: Flow V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A PLoS ONE, 2015;10(3):e0118499. Species: Human Sample Type: Whole Cells Application: ICC Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells. Authors: Nathwani B, Miller C, Yang T, Solimano J, Liao J Stem Cells Dev, 2014;23(2):115-23. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells. Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris PLoS ONE, 2014;9(12):e116114. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi Stem Cells Dev, 2014;23(18):2225-36. Species: Human Sample Type: Whole Cells Application: ICC Fresh Epigenetic reprogramming induces the expansion of cord blood stem cells. Authors: Chaurasia P, Gajzer D, Schaniel C, D'Souza S, Hoffman R J Clin Invest, 2014;124(6):2378-95. Species: Human Sample Type: Cell Lysates Application: IP Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells. Authors: Bershteyn, Marina, Hayashi, Yohei, Desachy, Guillaum, Hsiao, Edward C, Sami, Salma, Tsang, Kathryn, Weiss, Lauren A, Kriegstein, Arnold R, Yamanaka, Shinya, Wynshaw-Boris, Anthony Nature, 2014;507(7490):99-103. Species: Human Sample Type: Whole Cells Application: ICC Fresh Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome. Authors: Kobayashi, Junko, Yoshida, Masashi, Tarui, Suguru, Hirata, Masataka, Nagai, Yusuke, Kasahara, Shingo, Naruse, Keiji, Ito, Hiroshi, Sano, Shunji, Oh, Hidemasa PLoS ONE, 2014;9(7):e102796. Species: Human Sample Type: Whole Cells Application: ICC Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line. Authors: Wu, Yi-Lin, Ma, Shi-Rong, Peng, Tao, Teng, Zeng-Hui, Liang, Xiang-Ya, Guo, Guo-Zhen, Zhang, Hai-Feng, Li, Kang-Chu Int J Mol Sci, 2014;15(8):14180-90. Species: Human Sample Type: Cell Lysates Application: WB Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors. Authors: Piao Y, Hung S, Lim S, Wong R, Ko M Stem Cells Transl Med, 2014;3(0):787-791. Sox2 promotes tamoxifen resistance in breast cancer cells. Authors: Piva M, Domenici G, Iriondo O, Rabano M, Simoes B, Comaills V, Barredo I, Lopez-Ruiz J, Zabalza I, Kypta R, Vivanco M EMBO Mol Med, 2014;6(1):66-79. Species: Human Sample Type: Cell Lysates Application: WB A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium. Authors: Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima K, King R, Maminishkis A, Marugan J, Sharma R, Shure M, Temple S, Miller S, Bharti K Stem Cells Transl Med, 2014;3(8):911-22. Species: Human Sample Type: Whole Cells Application: IHC Fresh Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors. Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S PLoS ONE, 2014;9(1):e84547. Species: Human Sample Type: Whole Cells Application: ICC Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells. Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen Sci Rep, 2014;4(0):3779. Species: Human Sample Type: Cell Lysates Application: WB Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential. Authors: Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P Stem Cells Dev, 2013;22(23):3141-55. Species: Human Sample Type: Whole Cells Application: ICC Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome. Authors: Bellin, Milena, Casini, Simona, Davis, Richard, D'Aniello, Cristina, Haas, Jessica, Ward-van Oostwaard, Dorien, Tertoolen, Leon G J, Jung, Christia, Elliott, David A, Welling, Andrea, Laugwitz, Karl-Lud, Moretti, Alessand, Mummery, Christin EMBO J, 2013;32(24):3161-75. Species: Human Sample Type: Whole Cells Application: ICC The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients. Authors: Fatima A, Kaifeng S, Dittmann S, Xu G, Gupta M, Linke M, Zechner U, Nguemo F, Milting H, Farr M, Hescheler J, Saric T PLoS ONE, 2013;8(12):e83005. Species: Human Sample Type: Whole Cells Application: ICC Fresh Efficient generation of human iPSCs by a synthetic self-replicative RNA. Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S Cell Stem Cell, 2013;13(2):246-54. Species: Human Sample Type: Whole Cells Application: IF Differential L1 regulation in pluripotent stem cells of humans and apes. Authors: Marchetto M, Narvaiza I, Denli A, Benner C, Lazzarini T, Nathanson J, Paquola A, Desai K, Herai R, Weitzman M, Yeo G, Muotri A, Gage F Nature, 2013;503(7477):525-9. Species: Human Sample Type: Whole Cells Application: ICC A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells. Authors: Zhang R, Mjoseng H, Hoeve M, Bauer N, Pells S, Besseling R, Velugotla S, Tourniaire G, Kishen R, Tsenkina Y, Armit C, Duffy C, Helfen M, Edenhofer F, de Sousa P, Bradley M Nat Commun, 2013;4(0):1335. Species: Human Sample Type: Whole Cells Application: ICC Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji PLoS ONE, 2012;7(9):e45435. Species: Human Sample Type: Whole Cells Application: ICC Sox2 expression in breast tumours and activation in breast cancer stem cells. Authors: Leis O, Eguiara A, Lopez-Arribillaga E, Alberdi MJ, Hernandez-Garcia S, Elorriaga K, Pandiella A, Rezola R, Martin AG Oncogene, 2012;31(11):1354-65. Species: Human Sample Type: Whole Tissue Application: IHC Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells. Authors: Kim SY, Kim MJ, Jung H Stem Cells Dev., 2012;21(8):1272-86. Species: Human Sample Type: Whole Cells Application: ICC Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes. Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM Stem Cells Dev., 2012;21(3):404-10. Species: Human Sample Type: Whole Cells Application: ICC The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K Stem Cells Int, 2012;2012(0):875059. Species: Human Sample Type: Whole Cells Application: ICC CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H Stem Cells, 2011;29(4):609-17. Species: Human Sample Type: Whole Cells Application: ICC A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells. Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ Stem Cells, 2011;29(10):1517-27. Species: Human Sample Type: Cell Lysates Application: ChIP SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells. Authors: Chng Z, Teo A, Pedersen RA, Vallier L Cell Stem Cell, 2010;6(1):59-70. Species: Human Sample Type: Cell Lysates Application: ChIP A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Authors: Klim JR, Li L, Wrighton PJ Nat. Methods, 2010;7(12):989-94. Species: Human Sample Type: Whole Cells Application: ICC Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Authors: Chayosumrit M, Tuch B, Sidhu K Biomaterials, 2010;31(3):505-14. Species: Human Sample Type: Cell Lysates Application: WB Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome. Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL Nature, 2010;465(7299):808-12. Species: Human Sample Type: Whole Cells Application: ICC Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. Authors: Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J PLoS ONE, 2010;5(12):e14397. Species: Human Sample Type: Cell Lysates Application: WB Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells. Authors: Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K FEBS Lett., 2009;583(6):1029-33. Species: Human Sample Type: Whole Cells Application: ICC Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH FEBS Lett., 2009;583(3):561-7. Species: Human Sample Type: Whole Cells Application: ICC The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J. Cell. Biochem., 2009;106(5):867-77. Species: Human Sample Type: Whole Cells Application: ICC Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases. Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW Proc. Natl. Acad. Sci. U.S.A., 2009;106(24):9826-30. Species: Human Sample Type: Whole Cells Application: ICC Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C Biotechnol. Bioeng., 2009;102(6):1636-44. Species: Human Sample Type: Whole Cells Application: ICC High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L Cell Stem Cell, 2008;2(6):602-12. Species: Human Sample Type: Whole Cells Application: ICC Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S Cell, 2007;131(5):861-72. Species: Human Sample Type: Cell Lysates Application: WB Pedigreed primate embryonic stem cells express homogeneous familial gene profiles. Authors: Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G Stem Cells, 2007;25(11):2695-704. Species: Primate - Macaca mulatta (Rhesus Macaque) Sample Type: Whole Cells Application: ICC Isolation of a novel population of multipotent adult stem cells from human hair follicles. Authors: Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M, Xu X Am. J. Pathol., 2006;168(6):1879-88. Species: Human Sample Type: Whole Cells Application: ICC Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages. Authors: Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M Stem Cells, 2005;23(8):1035-43. Species: Human Sample Type: Cell Lysates Application: WB Monitoring differentiation of human embryonic stem cells using real-time PCR. Authors: Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, Kubista M, Sartipy P Stem Cells, 2005;23(10):1460-7. Species: Human Sample Type: Whole Cells Application: ICC Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells. Authors: Tsai MS, Hwang SM, Tsai YL, Cheng FC, Lee JL, Chang YJ Biol. Reprod., 2005;74(3):545-51. Species: Human Sample Type: Whole Cells Application: ICC Expand to show all Citations

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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Human Nanog Antibody

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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Goat IgG Horseradish Peroxidase-conjugated Antibody

Goat IgG (H+L) APC-conjugated Antibody

Donkey Anti-Goat IgG Antibody

Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

Goat IgG (H+L) Fluorescein-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

Chicken Anti-Goat IgG Biotinylated Antibody

Rabbit Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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