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Human Nanog Antibody

AF1997 102 Citations 5 Reviews Datasheet / COA / SDS
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AF1997
AF1997-SP
Detection of Human Nanog by Western Blot.
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Product Details
Citations (102)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nanog Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Nanog by Western Blot. View Larger

Detection of Human Nanog by Western Blot.
 Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

Chromatin Immunoprecipitation (ChIP) Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. View Larger

Detection of Nanog-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Nanog/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.

Immunocytochemistry Nanog in BG01V Human Stem Cells. View Larger

Nanog in BG01V Human Stem Cells. Nanog was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Nanog in BG01V Human Stem Cells-derived Embryoid Body. View Larger

Nanog in BG01V Human Stem Cells-derived Embryoid Body. Nanog was detected in immersion fixed BG01V human embryonic stem cells-derived embryoid body using 10 µg/mL Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Nanog in ADLF1 and FAB2 Stem Cell Lines. View Larger

Nanog in ADLF1 and FAB2 Stem Cell Lines. Nanog was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1-3).

References
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name
Nanog Homeobox
Entrez Gene IDs
79923 (Human); 71950 (Mouse)
Alternate Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

Product Datasheets

Product Datasheet
COA
SDS

Citations for Human Nanog Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

102 Citations: Showing 1 - 10
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  1. SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma
    Authors: M Sherman-Sa, H Onallah, A Holth, R Reich, B Davidson
    Gynecol. Oncol., 2019;0(0):.
    Species: Human
    Sample Types: Exosomes
    Applications: Western Blot
  2. Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients
    Authors: M Elkouris, G Kouroupi, A Vourvoukel, N Papagianna, V Kaltezioti, R Matsas, L Stefanis, M Xilouri, PK Politis
    Front Cell Neurosci, 2019;13(0):58.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype
    Authors: N Kolundzic, P Khurana, C Hobbs, M Rogar, S Ropret, H Törmä, D Ilic, M Liovic
    Stem Cell Res, 2019;37(0):101424.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient
    Authors: F Tsurumi, S Baba, D Yoshinaga, K Umeda, T Hirata, J Takita, T Heike
    PLoS ONE, 2019;14(3):e0213768.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development
    Authors: L Wang, AJ De Solis, Y Goffer, KE Birkenbach, SE Engle, R Tanis, JM Levenson, X Li, R Rausch, M Purohit, JY Lee, J Tan, MC De Rosa, CA Doege, HL Aaron, GJ Martins, JC Brüning, D Egli, R Costa, N Berbari, RL Leibel, G Stratigopo
    JCI Insight, 2019;4(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale
    Authors: X Wang, T Liao, C Wan, X Yang, J Zhao, R Fu, Z Yao, Y Huang, Y Shi, G Chang, Y Zheng, F Luo, Z Liu, Y Wang, X Mao, XY Zhao
    PeerJ, 2019;6(0):e6143.
    Species: Human
    Sample Types: Tissue Section
    Applications: IHC-Fr
  7. WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids
    Authors: A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Generation of a human induced pluripotent stem cell line from a patient with a rare A673T variant in amyloid precursor protein gene that reduces the risk for Alzheimer's disease
    Authors: Š Lehtonen, I Höytyläine, J Voutilaine, TM Sonninen, J Kuusisto, M Laakso, RH Hämäläinen, M Oksanen, J Koistinaho
    Stem Cell Res, 2018;30(0):96-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions
    Authors: RK Jangid, A Kelkar, VY Muley, S Galande
    BMC Genomics, 2018;19(1):313.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Preferential amplification of a human mitochondrial DNA deletion in vitro and in vivo
    Authors: OM Russell, I Fruh, PK Rai, D Marcellin, T Doll, A Reeve, M Germain, J Bastien, KA Rygiel, R Cerino, AW Sailer, M Lako, RW Taylor, M Mueller, RN Lightowler, DM Turnbull, SB Helliwell
    Sci Rep, 2018;8(1):1799.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Biological characterization of human amniotic epithelial cells in a serum-free system and their safety evaluation
    Authors: PJ Yang, WX Yuan, J Liu, JY Li, B Tan, C Qiu, XL Zhu, C Qiu, DM Lai, LH Guo, LY Yu
    Acta Pharmacol. Sin., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. DMSO increases efficiency of genome editing at two non-coding loci
    Authors: G Stratigopo, MC De Rosa, CA LeDuc, RL Leibel, CA Doege
    PLoS ONE, 2018;13(6):e0198637.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. High-efficiency RNA-based reprogramming of human primary fibroblasts
    Authors: I Kogut, SM McCarthy, M Pavlova, DP Astling, X Chen, A Jakimenko, KL Jones, A Getahun, JC Cambier, AMG Pasmooij, MF Jonkman, DR Roop, G Bilousova
    Nat Commun, 2018;9(1):745.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Generation of gene-corrected iPSC line from Parkinson's disease patient iPSC line with alpha-SNCA A53T mutation
    Authors: SY Lee, S Jeong, J Kim, SK Chung
    Stem Cell Res, 2018;30(0):145-149.
  15. TFAP2C regulates transcription in human naive pluripotency by opening enhancers
    Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
    Nat. Cell Biol., 2018;20(5):553-564.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Functional Dissection of the Enhancer Repertoire in Human Embryonic Stem Cells
    Authors: TS Barakat, F Halbritter, M Zhang, AF Rendeiro, E Perenthale, C Bock, I Chambers
    Cell Stem Cell, 2018;23(2):276-288.e8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  17. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018;7(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A
    Authors: L Devito, M Donne, N Kolundzic, P Khurana, C Hobbs, G Kaddour, S Dubrac, R Gruber, M Schmuth, T Mauro, D Ilic
    Stem Cell Res, 2018;31(0):122-126.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys
    Authors: Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li
    Cell Rep, 2018;25(9):2563-2576.e9.
    Species: Primate - cynomolgus
    Sample Types: Whole Cell
    Applications: ICC
  20. 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling
    Authors: Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: immunohistochemistry
  21. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Differentiation
  22. yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs
    Authors: S Frank, G Ahuja, D Bartsch, N Russ, W Yao, JC Kuo, JP Derks, VS Akhade, Y Kargapolov, T Georgomano, JE Messling, M Gramm, L Brant, R Rehimi, NE Vargas, A Kuroczik, TP Yang, RGA Sahito, J Franzen, J Hescheler, A Sachinidis, M Peifer, A Rada-Igles, M Kanduri, IG Costa, C Kanduri, A Papantonis, L Kurian
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  23. Generation of induced pluripotent stem cells (iPSCs) from human foreskin fibroblasts
    Authors: F Erdlenbruc, J Rohwedel, P Ralfs, A Thomitzek, J Kramer, F Cakiroglu
    Stem Cell Res, 2018;33(0):79-82.
    Species: Human
    Sample Types: Whole Cell
    Applications: ICC
  24. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation
    Authors: CA Thomas, L Tejwani, CA Trujillo, PD Negraes, RH Herai, P Mesci, A Macia, YJ Crow, AR Muotri
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9
    Authors: Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li
    Stem Cell Res, 2017;22(0):29-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status
    Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda
    Stem Cells Int, 2017;2017(0):7906843.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders
    Authors: C Lorenz, P Lesimple, R Bukowiecki, A Zink, G Inak, B Mlody, M Singh, M Semtner, N Mah, K Aur‚, M Leong, O Zabiegalov, EM Lyras, V Pfiffer, B Fauler, J Eichhorst, B Wiesner, N Huebner, J Priller, T Mielke, D Meierhofer, Z Izsv k, JC Meier, F Bouillaud, J Adjaye, M Schuelke, EE Wanker, A LombŠs, A Prigione
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)
    Authors: E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés
    Stem Cell Res, 2017;21(0):19-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration
    Authors: JS Saini, B Corneo, JD Miller, TR Kiehl, Q Wang, NC Boles, TA Blenkinsop, JH Stern, S Temple
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. TALEN-mediated shift of mitochondrial DNA heteroplasmy in MELAS-iPSCs with m.13513G>A mutation
    Authors: N Yahata, Y Matsumoto, M Omi, N Yamamoto, R Hata
    Sci Rep, 2017;7(1):15557.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
    Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
    Cell Death Discov, 2017;3(0):16105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells
    Authors: E Baljinnyam, S Venkatesh, R Gordan, S Mareedu, J Zhang, LH Xie, EI Azzam, CK Suzuki, D Fraidenrai
    Physiol Rep, 2017;5(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  34. Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing
    Authors: G Xu, D Guo, F Wu, N Abbas, K Lai, F Yuan, K You, Y Liu, Y Zhuang, Y Wu, Y Xu, Y Chen, F Yang, T Pan, YX Li
    Stem Cell Res, 2017;27(0):38-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Differentiation-Defective Human Induced Pluripotent Stem Cells Reveal�Strengths and Limitations of the Teratoma Assay and In�Vitro Pluripotency Assays
    Authors: MJ Bouma, M van Iterso, B Janssen, CL Mummery, DCF Salvatori, C Freund
    Stem Cell Reports, 2017;8(5):1340-1353.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  36. A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling
    Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel
    Stem Cell Res, 2017;23(0):73-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS)
    Authors: B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya
    Stem Cell Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation
    Authors: CW Chang, CC Chang, KC Hsia, SY Tsai
    Stem Cell Res, 2017;27(0):21-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines
    Authors: L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya
    Stem Cell Res, 2017;24(0):155-159.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
    Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
    Circ. Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
    Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
    Nat Protoc, 2017;12(4):814-827.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming
    Authors: C Eguizabal
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  43. Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells
    Stem Cell Reports, 2016;7(3):496-507.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  44. Generation of HEXA-deficient hiPSCs from fibroblasts of a Tay-Sachs disease patient
    Stem Cell Res, 2016;17(2):289-291.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Fresh
  45. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts
    Stem Cell Res, 2016;17(2):444-447.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: IHC Fresh
  46. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
    PLoS ONE, 2016;11(9):e0161229.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: IHC Not Specified
  47. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
    Authors: N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
    Nat. Med., 2016;22(12):1421-1427.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016;11(4):e0152993.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  49. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients
    Elife, 2016;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  50. Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization
    Authors: Florian Wegner
    J. Neurosci., 2016;36(47):12027-12043.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application
    Stem Cell Res, 2016;17(2):379-390.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC PFA fixed
  52. Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow
    FEBS Open Bio, 2016;6(11):1054-1066.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
    Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A
    PLoS ONE, 2015;10(3):e0118499.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. beta-Catenin and NF-kappaB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer.
    Authors: Jia D, Yang W, Li L, Liu H, Tan Y, Ooi S, Chi L, Filion L, Figeys D, Wang L
    Cell Death Differ, 2015;22(2):298-310.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  55. Metabolic rescue in pluripotent cells from patients with mtDNA disease.
    Authors: Ma H, Folmes C, Wu J, Morey R, Mora-Castilla S, Ocampo A, Ma L, Poulton J, Wang X, Ahmed R, Kang E, Lee Y, Hayama T, Li Y, Van Dyken C, Gutierrez N, Tippner-Hedges R, Koski A, Mitalipov N, Amato P, Wolf D, Huang T, Terzic A, Laurent L, Izpisua Belmonte J, Mitalipov S
    Nature, 2015;524(7564):234-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Not specified
  56. GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport.
    Authors: Freibaum B, Lu Y, Lopez-Gonzalez R, Kim N, Almeida S, Lee K, Badders N, Valentine M, Miller B, Wong P, Petrucelli L, Kim H, Gao F, Taylor J
    Nature, 2015;525(7567):129-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  57. Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer.
    Authors: Francescangeli F, Contavalli P, De Angelis M, Baiocchi M, Gambara G, Pagliuca A, Fiorenzano A, Prezioso C, Boe A, Todaro M, Stassi G, Castro N, Watanabe K, Salomon D, De Maria R, Minchiotti G, Zeuner A
    Cell Death Differ, 2015;22(10):1700-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  58. Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
    Authors: Liu L, Wu L, Wei X, Ling J
    Stem Cells Dev, 2015;24(8):962-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fluorescence
  59. Morphological differences of primary cilia between human induced pluripotent stem cells and their parental somatic cells.
    Authors: Nathwani B, Miller C, Yang T, Solimano J, Liao J
    Stem Cells Dev, 2014;23(2):115-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fluorescence
  60. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014;23(18):2225-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fresh
  61. Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors.
    Authors: Piao Y, Hung S, Lim S, Wong R, Ko M
    Stem Cells Transl Med, 2014;3(0):787-791.
  62. Epigenetic reprogramming induces the expansion of cord blood stem cells.
    Authors: Chaurasia P, Gajzer D, Schaniel C, D'Souza S, Hoffman R
    J Clin Invest, 2014;124(6):2378-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  63. Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
    Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris
    PLoS ONE, 2014;9(12):e116114.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fluorescence
  64. Directed differentiation of patient-specific induced pluripotent stem cells identifies the transcriptional repression and epigenetic modification of NKX2-5, HAND1, and NOTCH1 in hypoplastic left heart syndrome.
    Authors: Kobayashi, Junko, Yoshida, Masashi, Tarui, Suguru, Hirata, Masataka, Nagai, Yusuke, Kasahara, Shingo, Naruse, Keiji, Ito, Hiroshi, Sano, Shunji, Oh, Hidemasa
    PLoS ONE, 2014;9(7):e102796.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  65. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014;4(0):3779.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  66. Effects of pulsed electromagnetic field on differentiation of HUES-17 human embryonic stem cell line.
    Authors: Wu, Yi-Lin, Ma, Shi-Rong, Peng, Tao, Teng, Zeng-Hui, Liang, Xiang-Ya, Guo, Guo-Zhen, Zhang, Hai-Feng, Li, Kang-Chu
    Int J Mol Sci, 2014;15(8):14180-90.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  67. Sox2 promotes tamoxifen resistance in breast cancer cells.
    Authors: Piva M, Domenici G, Iriondo O, Rabano M, Simoes B, Comaills V, Barredo I, Lopez-Ruiz J, Zabalza I, Kypta R, Vivanco M
    EMBO Mol Med, 2014;6(1):66-79.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  68. Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
    Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
    PLoS ONE, 2014;9(1):e84547.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.
    Authors: Ferrer M, Corneo B, Davis J, Wan Q, Miyagishima K, King R, Maminishkis A, Marugan J, Sharma R, Shure M, Temple S, Miller S, Bharti K
    Stem Cells Transl Med, 2014;3(8):911-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Fresh
  70. Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
    Authors: Bershteyn, Marina, Hayashi, Yohei, Desachy, Guillaum, Hsiao, Edward C, Sami, Salma, Tsang, Kathryn, Weiss, Lauren A, Kriegstein, Arnold R, Yamanaka, Shinya, Wynshaw-Boris, Anthony
    Nature, 2014;507(7490):99-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fresh
  71. Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome.
    Authors: Bellin, Milena, Casini, Simona, Davis, Richard, D'Aniello, Cristina, Haas, Jessica, Ward-van Oostwaard, Dorien, Tertoolen, Leon G J, Jung, Christia, Elliott, David A, Welling, Andrea, Laugwitz, Karl-Lud, Moretti, Alessand, Mummery, Christin
    EMBO J, 2013;32(24):3161-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  72. Differential L1 regulation in pluripotent stem cells of humans and apes.
    Authors: Marchetto M, Narvaiza I, Denli A, Benner C, Lazzarini T, Nathanson J, Paquola A, Desai K, Herai R, Weitzman M, Yeo G, Muotri A, Gage F
    Nature, 2013;503(7477):525-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  73. Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
    Authors: Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P
    Stem Cells Dev, 2013;22(23):3141-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  74. The disease-specific phenotype in cardiomyocytes derived from induced pluripotent stem cells of two long QT syndrome type 3 patients.
    Authors: Fatima A, Kaifeng S, Dittmann S, Xu G, Gupta M, Linke M, Zechner U, Nguemo F, Milting H, Farr M, Hescheler J, Saric T
    PLoS ONE, 2013;8(12):e83005.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC Fresh
  75. A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells.
    Authors: Zhang R, Mjoseng H, Hoeve M, Bauer N, Pells S, Besseling R, Velugotla S, Tourniaire G, Kishen R, Tsenkina Y, Armit C, Duffy C, Helfen M, Edenhofer F, de Sousa P, Bradley M
    Nat Commun, 2013;4(0):1335.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  76. Efficient generation of human iPSCs by a synthetic self-replicative RNA.
    Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S
    Cell Stem Cell, 2013;13(2):246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunofluorescence
  77. The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells.
    Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K
    Stem Cells Int, 2012;2012(0):875059.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  79. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2012;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  80. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2012;21(8):1272-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  81. Sox2 expression in breast tumours and activation in breast cancer stem cells.
    Authors: Leis O, Eguiara A, Lopez-Arribillaga E, Alberdi MJ, Hernandez-Garcia S, Elorriaga K, Pandiella A, Rezola R, Martin AG
    Oncogene, 2012;31(11):1354-65.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  82. CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells.
    Authors: Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H
    Stem Cells, 2011;29(4):609-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  83. A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
    Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
    Stem Cells, 2011;29(10):1517-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  84. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  85. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
    Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL
    Nature, 2010;465(7299):808-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  86. Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach.
    Authors: Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J
    PLoS ONE, 2010;5(12):e14397.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  87. Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm.
    Authors: Chayosumrit M, Tuch B, Sidhu K
    Biomaterials, 2010;31(3):505-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  88. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  89. Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.
    Authors: Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(24):9826-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  90. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  91. The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
    J. Cell. Biochem., 2009;106(5):867-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  92. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
    Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
    Biotechnol. Bioeng., 2009;102(6):1636-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  93. Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
    Authors: Taura D, Noguchi M, Sone M, Hosoda K, Mori E, Okada Y, Takahashi K, Homma K, Oyamada N, Inuzuka M, Sonoyama T, Ebihara K, Tamura N, Itoh H, Suemori H, Nakatsuji N, Okano H, Yamanaka S, Nakao K
    FEBS Lett., 2009;583(6):1029-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  94. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells.
    Authors: Desbordes SC, Placantonakis DG, Ciro A, Socci ND, Lee G, Djaballah H, Studer L
    Cell Stem Cell, 2008;2(6):602-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  95. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
    Cell, 2007;131(5):861-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  96. Pedigreed primate embryonic stem cells express homogeneous familial gene profiles.
    Authors: Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G
    Stem Cells, 2007;25(11):2695-704.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: ICC
  97. Isolation of a novel population of multipotent adult stem cells from human hair follicles.
    Authors: Yu H, Fang D, Kumar SM, Li L, Nguyen TK, Acs G, Herlyn M, Xu X
    Am. J. Pathol., 2006;168(6):1879-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  98. Monitoring differentiation of human embryonic stem cells using real-time PCR.
    Authors: Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, Kubista M, Sartipy P
    Stem Cells, 2005;23(10):1460-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  99. Clonal amniotic fluid-derived stem cells express characteristics of both mesenchymal and neural stem cells.
    Authors: Tsai MS, Hwang SM, Tsai YL, Cheng FC, Lee JL, Chang YJ
    Biol. Reprod., 2005;74(3):545-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  100. Downregulation of NANOG induces differentiation of human embryonic stem cells to extraembryonic lineages.
    Authors: Hyslop L, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M
    Stem Cells, 2005;23(8):1035-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  101. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.
    Authors: Shafa M, Yang F, Fellner T, Rao M, Baghbaderani B
    Front Med (Lausanne), 0;5(0):69.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human Nanog Antibody
By Anonymous on 04/13/2018
Application: WB Sample Tested: Tumor cell lyastes Species: Human
Human Nanog Antibody
By Anonymous on 11/23/2017
Application: ICC/IF Sample Tested: Embryonic stem cells Species: Human
Immunocytochemistry/Immunofluorescence Human Nanog Antibody AF1997
Human Nanog Antibody
By Anonymous on 07/05/2017
Application: WB Sample Tested: 7TD1 mouse hybridoma cell line Species: Mouse
Human Nanog Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: stem cells Species: Human

Works nicely in immunostainings 1:50.

Human Nanog Antibody
By Anonymous on 12/15/2016
Application: WB Sample Tested: stem cells Species: Human

Works nicely in Western blotting 1:5000.