Human Neurocan Antibody

Catalog # Availability Size / Price Qty
AF6508
AF6508-SP
Human Neurocan Antibody in Immunohistochemistry
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Neurocan Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neurocan in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Neurocan is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Neurocan
Glu23-Cys1321
Accession # O14594
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Neurocan in Human Brain. View Larger

Neurocan in Human Brain. Neurocan was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6508) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurocan

Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C‑type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and C‑terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6-8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10-15).

References
  1. Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842.
  2. Prange, C.K. et al. (1998) Gene 221:199.
  3. Rauch, U. et al. (1991) J. Biol. Chem. 266:14785.
  4. Matsui, F. et al. (1994) Neurochem. Int. 25:425.
  5. Rauch, U. et al. (1992) J. Biol. Chem. 267:19536.
  6. Asher, R.A. et al. (2000) J. Neurosci. 20:2427.
  7. Matsui, F. et al. (1998) Brain Res. 790:45.
  8. Abaskharoun, M. et al. (2010) Brain Res. 1327:6.
  9. Shen, L.H. et al. (2008) Glia 56:1747.
  10. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  11. Sango, K. et al. (2003) Exp. Neurol. 182:1.
  12. Akita, K. et al. (2004) Biochem. J. 383:129.
  13. Grumet, M. et al. (1994) J. Biol. Chem. 269:12142.
  14. Milev, P. et al. (1996) J. Biol. Chem. 271:15716.
  15. Rauch, U. et al. (2004) Matrix Biol. 22:629.
Entrez Gene IDs
1463 (Human); 13004 (Mouse); 58982 (Rat)
Alternate Names
chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; Neurocan

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Neurocan Antibody

There are currently no reviews for this product. Be the first to review Human Neurocan Antibody and earn rewards!

Have you used Human Neurocan Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review