Human Neurocan Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Neurocan in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Neurocan is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Neurocan
    Glu23-Cys1321
    Accession # O14594
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Neurocan in Human Brain. Neurocan was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6508) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurocan
Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C‑type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and C‑terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6-8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10-15).
  • References:
    1. Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842.
    2. Prange, C.K. et al. (1998) Gene 221:199.
    3. Rauch, U. et al. (1991) J. Biol. Chem. 266:14785.
    4. Matsui, F. et al. (1994) Neurochem. Int. 25:425.
    5. Rauch, U. et al. (1992) J. Biol. Chem. 267:19536.
    6. Asher, R.A. et al. (2000) J. Neurosci. 20:2427.
    7. Matsui, F. et al. (1998) Brain Res. 790:45.
    8. Abaskharoun, M. et al. (2010) Brain Res. 1327:6.
    9. Shen, L.H. et al. (2008) Glia 56:1747.
    10. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
    11. Sango, K. et al. (2003) Exp. Neurol. 182:1.
    12. Akita, K. et al. (2004) Biochem. J. 383:129.
    13. Grumet, M. et al. (1994) J. Biol. Chem. 269:12142.
    14. Milev, P. et al. (1996) J. Biol. Chem. 271:15716.
    15. Rauch, U. et al. (2004) Matrix Biol. 22:629.
  • Entrez Gene IDs:
    1463 (Human); 13004 (Mouse); 58982 (Rat)
  • Alternate Names:
    chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; Neurocan
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