Human Neuroligin 3/NLGN3 Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Neuroligin 3/NLGN3

NLGN3 (Neuroligin 3; also gliotactin homolog) is a 110-114 kDa member of the type-B carboxyesterase/lipase family of proteins.  It is a neuronal transmembrane protein that forms Ca⁺⁺-dependent intercellular junctions with short beta -neurexin isoforms.  This seems to contribute to both glutamatergic and GABAergic synapse formation.  Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism.  Mature human NLGN3 is an 811 amino acid (aa) type I transmembrane protein.  It contains a 672 aa extracellular domain (ECD) (aa 38-709), plus a 118 aa cytoplasmic region.  The ECD possesses a nonfunctional carboxyesterase domain (aa 41-625).  Multiple splice variants exist.  There is a deletion of aa 153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa 153-192 that may also be accompanied by a five Lys substitution for aa 554-848.  Over aa 38-709, human NLGN3 shares 99% aa identity with mouse NLGN3.        

Product Datasheets

Related Research Areas

• Neurexins and Neuroligins
• Synaptic Adhesion Molecules