Human Neuropilin-1 Quantikine ELISA Kit

R&D Systems | Catalog # DNRP10

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (13 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (10 µL)

Sensitivity

9.33 pg/mL

Assay Range

78.1-5000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
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Product Summary for Human Neuropilin-1 Quantikine ELISA Kit

The Quantikine Human Neuropilin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Neuropilin-1 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Neuropilin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Neuropilin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Neuropilin-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Neuropilin-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 336 967 1890 329 934 1833
Standard Deviation 17.9 70.4 54.7 19.8 48.4 90.4
CV% 5.3 7.3 2.9 6.0 5.2 4.9

Recovery for Human Neuropilin-1 Quantikine ELISA Kit

The recovery of human Neuropilin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 91 86-99
Cell Lysates (n=3) 101 94-108
Urine (n=4) 99 85-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Neuropilin-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Neuropilin-1 ELISA Linearity

Scientific Data Images for Human Neuropilin-1 Quantikine ELISA Kit

Human Neuropilin-1 ELISA Standard Curve

Human Neuropilin-1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuropilin-1

Neuropilin-1 (NRP1) is a transmembrane glycoprotein that regulates axon guidance and angiogenesis. Distinct Neuropilin-1 domains mediate its binding to heparin, the heparin-binding forms of VEGF and PlGF (PlGF2), and Semaphorin 3. Neuropilin-1 can form homo- and hetero-oligomers with Neuropilin-2. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. They mediate axon repulsion on sensory and sympathetic neurons through binding to Class III Semaphorins, Plexin A3, and Plexin A4.  They also associate with VEGF R2 and function as coreceptors for VEGF. NRP1 is preferentially expressed in developing or remodeling arteries, epithelial cells, and carcinomas, while Neuropilin-2 is primarily found in veins and lymphatics. Neuroplin-1 is additionally expressed on dendritic cells where it mediates DC-induced T cell proliferation. NRP1 also plays a role in SARS-CoV-2 infection in humans, the virus responsible for COVID-19. The Spike protein of SARS-CoV-2 can bind to the b1 domain of Neuropilin-1 on the cell surface initiating viral entry into the host cell. Blocking the NRP1-Spike interaction reduces viral infectivity in vitro making it a potential therapeutic target. 

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

Additional Neuropilin-1 Products

Product Documents for Human Neuropilin-1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Neuropilin-1 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Neuropilin-1 Quantikine ELISA Kit

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  • Human Neuropilin-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma and Urine
    Verified Customer | Posted 11/03/2023
    Measured plasma at 200-fold dilution ad measured urine undiluted. We were able to obtain values in range in both biofluids.
    Human Neuropilin-1 Quantikine ELISA Kit DNRP10

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Protocols

View specific protocols for Human Neuropilin-1 Quantikine ELISA Kit (DNRP10):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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