Human NG2/MCSP APC-conjugated Antibody

Catalog #: FAB2585A
3 Citations Datasheet / COA / SDS

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FAB2585A

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Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NG2/MCSP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # LHM-2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human melanoma cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP APC-conjugated Monoclonal Antibody (Catalog # FAB2585A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References

Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
Legg, J. et al. (2003) Development 130:6049.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Alternate Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human NG2/MCSP APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells
Authors: G MacLeod, DA Bozek, N Rajakulend, V Monteiro, M Ahmadi, Z Steinhart, MM Kushida, H Yu, FJ Coutinho, FMG Cavalli, I Restall, X Hao, T Hart, HA Luchman, S Weiss, PB Dirks, S Angers
Cell Rep, 2019;27(3):971-986.e9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
Stem Cells Dev., 2017;0(0):.
Species: Equine
Sample Types: Whole Cells
Applications: Flow Cytometry
Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
, 2014;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry