Human NG2/MCSP PE-conjugated Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NG2/MCSP in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # LHM-2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human melanoma cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP PE-conjugated Monoclonal Antibody (Catalog # FAB2585P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NG2/MCSP
NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).
  • References:
    1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
    2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
    3. Legg, J. et al. (2003) Development 130:6049.
  • Long Name:
    Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
  • Entrez Gene IDs:
    1464 (Human); 121021 (Mouse); 81651 (Rat)
  • Alternate Names:
    AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.
    Authors: Yuan K, Orcholski M, Panaroni C, Shuffle E, Huang N, Jiang X, Tian W, Vladar E, Wang L, Nicolls M, Wu J, de Jesus Perez V
    Am J Pathol, 2015;185(1):69-84.
    Species: Human
    Sample Type: Whole Cells
    Application: cell sorting
  2. Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts.
    Authors: Orlova, Valeria, Drabsch, Yvette, Freund, Christia, Petrus-Reurer, Sandra, van den Hil, Francijn, Muenthaisong, Suchitra, Dijke, Peter Te, Mummery, Christin
    Arterioscler Thromb Vasc Biol, 2014;34(1):177-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB signaling pathway and proinflammatory response in human pericytes.
    Authors: Guijarro-Munoz I, Compte M, Alvarez-Cienfuegos A, Alvarez-Vallina L, Sanz L
    J Biol Chem, 2014;289(4):2457-68.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
    Authors: Cheung C, Bernardo AS, Trotter MW
    Nat. Biotechnol., 2012;30(2):165-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2012;318(4):371-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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