Human NG2/MCSP PE-conjugated Antibody

Catalog #: FAB2585P
6 Citations Datasheet / COA / SDS

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FAB2585P

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Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry.

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Citations (6)

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Human NG2/MCSP PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NG2/MCSP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # LHM-2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human melanoma cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP PE-conjugated Monoclonal Antibody (Catalog # FAB2585P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References

Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
Legg, J. et al. (2003) Development 130:6049.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Alternate Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human NG2/MCSP PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head
Authors: M Herrmann, M Hildebrand, U Menzel, N Fahy, M Alini, S Lang, L Benneker, S Verrier, MJ Stoddart, JJ Bara
Int J Mol Sci, 2019-07-14;20(14):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis.
Authors: Yuan K, Orcholski M, Panaroni C, Shuffle E, Huang N, Jiang X, Tian W, Vladar E, Wang L, Nicolls M, Wu J, de Jesus Perez V
Am J Pathol, 2014-11-06;185(1):69-84.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection
Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB signaling pathway and proinflammatory response in human pericytes.
Authors: Guijarro-Munoz I, Compte M, Alvarez-Cienfuegos A, Alvarez-Vallina L, Sanz L
J Biol Chem, 2013-12-04;289(4):2457-68.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts.
Authors: Orlova, Valeria, Drabsch, Yvette, Freund, Christia, Petrus-Reurer, Sandra, van den Hil, Francijn, Muenthaisong, Suchitra, Dijke, Peter Te, Mummery, Christin
Arterioscler Thromb Vasc Biol, 2013-10-24;34(1):177-86.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
Authors: Cheung C, Bernardo AS, Trotter MW
Nat. Biotechnol., 2012-01-15;30(2):165-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
Exp. Cell Res., 2011-12-14;318(4):371-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry