Human NG2/MCSP PE‑conjugated Antibody

R&D Systems | Catalog # FAB2585P

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Cell Selection

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # LHM-2
View all NG2/MCSP Primary Antibodies »

Product Specifications

Immunogen

Human melanoma cells

Specificity

Detects human NG2/MCSP in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NG2/MCSP PE‑conjugated Antibody

Detection of NG2/MCSP in A375 cells by Flow Cytometry

A375 cells were stained with Mouse Anti-Human NG2/MCSP PE‑conjugated Monoclonal Antibody (Catalog # FAB2585P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of NG2/MCSP antibody in G361 Human Cell Line antibody by Flow Cytometry.

Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry.

G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP PE-conjugated Monoclonal Antibody (Catalog # FAB2585P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human NG2/MCSP PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: A375 and G361 human melanoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References

  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Gene Symbol

CSPG4

Additional NG2/MCSP Products

Product Documents for Human NG2/MCSP PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NG2/MCSP PE‑conjugated Antibody

For research use only

Citations for Human NG2/MCSP PE‑conjugated Antibody

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