Human Nidogen-1/Entactin Antibody

Catalog # Availability Size / Price Qty
AF2570
AF2570-SP
Human Nidogen-1/Entactin Antibody in Data
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Product Details
Citations (9)
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Reviews (1)

Human Nidogen-1/Entactin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nidogen‑1/Entactin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nidogen‑1/Entactin
Leu29-Lys1114 (Gln1113Arg)
Accession # AAH45606
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Nidogen‑1/Entactin (Catalog # 2570-ND)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Nidogen‑1/Entactin antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Nidogen‑1/Entactin in Human Heart. Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Nidogen‑1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nidogen-1/Entactin

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.

References
  1. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115. 
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  4. Timpl, R. and J.C. Brown (1996) BioEssays 18:123. 
  5. Nagayoshi, T. et al. (1989) DNA 8:581. 
  6. Zimmerman, K. et al. (1995) Genomics 27:245. 
  7. Fox, J.W. et al. (1991) EMBO J. 10:3137.
  8. Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
  9. Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
  10. Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
  11. Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
  12. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
Entrez Gene IDs
4811 (Human)
Alternate Names
enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; Nidogen1; Nidogen-1

Product Datasheets

Citations for Human Nidogen-1/Entactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. BET protein inhibitor JQ1 downregulates chromatin accessibility and suppresses metastasis of gastric cancer via inactivating RUNX2/NID1 signaling
    Authors: S Zhou, S Zhang, L Wang, S Huang, Y Yuan, J Yang, H Wang, X Li, P Wang, L Zhou, J Yang, Y Xu, H Gao, Y Zhang, Y Lv, X Zou
    Oncogenesis, 2020;9(3):33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Descemet's Membrane Modulation of Posterior Corneal Fibrosis
    Authors: CS Medeiros, P Saikia, RC de Oliveir, L Lassance, MR Santhiago, SE Wilson
    Invest. Ophthalmol. Vis. Sci., 2019;60(4):1010-1020.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Immune-mediated ECM depletion improves tumour perfusion and payload delivery
    Authors: YL Yeow, VR Kotamraju, X Wang, M Chopra, N Azme, J Wu, TD Schoep, DS Delaney, K Feindel, J Li, KM Kennedy, WM Allen, BF Kennedy, I Larma, DD Sampson, LM Mahakian, BZ Fite, H Zhang, T Friman, AP Mann, FA Aziz, MP Kumarasing, M Johansson, HC Ee, G Yeoh, L Mou, KW Ferrara, H Billiran, R Ganss, E Ruoslahti, J Hamzah
    EMBO Mol Med, 2019;0(0):e10923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. IL-1 and TGF-? Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells
    Authors: P Saikia, S Thangavadi, CS Medeiros, L Lassance, RC de Oliveir, SE Wilson
    Invest. Ophthalmol. Vis. Sci., 2018;59(13):5589-5598.
    Species: Rabbit
    Sample Types: Cell Extracts
    Applications: Western Blot
  5. Posterior stromal cell apoptosis triggered by mechanical endothelial injury and basement membrane component nidogen-1 production in the cornea
    Authors: CS Medeiros, L Lassance, P Saikia, M Santhiago, SE Wilson
    Exp. Eye Res., 2018;0(0):.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: IHC
  6. Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity.
    Authors: Mattsson J, Ravela S, Hekim C, Jonsson M, Malm J, Narvanen A, Stenman U, Koistinen H
    PLoS ONE, 2014;9(9):e107819.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  7. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Development and characterization of a high-throughput in vitro cord formation model insensitive to VEGF inhibition.
    Authors: Falcon B, O'Clair B, McClure D, Evans G, Stewart J, Swearingen M, Chen Y, Allard K, Lee L, Neote K, McEwen D, Uhlik M, Chintharlapalli S
    J Hematol Oncol, 2013;6(0):31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
    Authors: Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE
    Blood, 2009;114(24):5091-101.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Nidogen-1/Entactin Antibody
By Anonymous on 05/08/2018
Application: Very specific antibody! Sample Tested: Eye lens tissue Species: Rabbit