Human Nidogen-1/Entactin Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Nidogen‑1/Entactin in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Nidogen‑1/Entactin
    Leu29-Lys1114 (Gln1113Arg)
    Accession # AAH45606
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Nidogen‑1/Entactin (Catalog # 2570-ND)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Nidogen‑1/Entactin in Human Heart. Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Nidogen‑1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nidogen-1/Entactin

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.

  • References:
    1. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115. 
    2. Miosge, N. et al. (2001) Histochem. J. 33:523.
    3. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
    4. Timpl, R. and J.C. Brown (1996) BioEssays 18:123. 
    5. Nagayoshi, T. et al. (1989) DNA 8:581. 
    6. Zimmerman, K. et al. (1995) Genomics 27:245. 
    7. Fox, J.W. et al. (1991) EMBO J. 10:3137.
    8. Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
    9. Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
    10. Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
    11. Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
    12. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
  • Entrez Gene IDs:
    4811 (Human)
  • Alternate Names:
    enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; Nidogen1; Nidogen-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity.
    Authors: Mattsson J, Ravela S, Hekim C, Jonsson M, Malm J, Narvanen A, Stenman U, Koistinen H
    PLoS ONE, 2014;9(9):e107819.
    Species: Human
    Sample Type: Protein
    Application: WB
  2. Development and characterization of a high-throughput in vitro cord formation model insensitive to VEGF inhibition.
    Authors: Falcon B, O'Clair B, McClure D, Evans G, Stewart J, Swearingen M, Chen Y, Allard K, Lee L, Neote K, McEwen D, Uhlik M, Chintharlapalli S
    J Hematol Oncol, 2013;6(0):31.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  3. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013;8(12):e85147.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  4. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
    Authors: Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE
    Blood, 2009;114(24):5091-101.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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