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Human NIK/MAP3K14 Antibody

Catalog #: MAB6888 1 Review Datasheet / COA / SDS

Discontinued Product

MAB6888 has been discontinued.
View all NIK/MAP3K14 products.
NIK/MAP3K14 in Human Pancreas.
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Human NIK/MAP3K14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NIK/MAP3K14 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 603917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NIK/MAP3K14
Glu769-Pro947
Accession # Q99558
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry NIK/MAP3K14 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

NIK/MAP3K14 in Human Pancreas. NIK/MAP3K14 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human NIK/MAP3K14 Monoclonal Antibody (Catalog # MAB6888) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NIK/MAP3K14

NIK (NF kappa B inducing kinase), also called MAP3K14, is a widely expressed 947 amino acid (aa), ~100 kDa cytoplasmic protein of the MAP kinase family. NIK participates in a non-canonical NFkB signaling cascade in which its activity and expression are elevated by TNF and related ligands. NIK activates IKKa/b, which releases NFkB subunits for translocation to the nucleus. Within the region used as an immunogen, human NIK shares 94% and 95% aa sequence identity with mouse and rat NIK, respectively.

Long Name
NF-kappa-beta Inducing Kinase
Entrez Gene IDs
9020 (Human); 53859 (Mouse); 360640 (Rat)
Alternate Names
EC 2.7.11.25; FTDCR1B; HS; HSNIK; HSNIKserine/threonine protein-kinase; MAP3K14; mitogen-activated protein kinase kinase kinase 14; NIK; NIKNF-kappa-beta-inducing kinase; Serine/threonine-protein kinase NIK

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Reviews for Human NIK/MAP3K14 Antibody

Average Rating: 4 (Based on 1 Review)

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Human NIK/MAP3K14 Antibody
By Anonymous on 07/16/2020
Application: ELISA Sample Tested: Cancer cell lysates Species: Human
ELISA Human NIK/MAP3K14 Antibody MAB6888