Human NKp30/NCR3 Alexa Fluor® 488-conjugated Antibody

Clone 210845 was used by HLDA to establish CD designation.
  • Species Reactivity
  • Specificity
    Detects human NKp30/NCR3 on cell transfectants and peripheral blood NK cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 210845
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse T cell hybridoma transfected with human NKp30/NCR3 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKp30/NCR3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKp30/NCR3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1849G) or (B) Mouse IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC003G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
    4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
  • Entrez Gene IDs:
    259197 (Human)
  • Alternate Names:
    Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKP30; NKp30MALS
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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