Human NKp30/NCR3 Antibody

R&D Systems | Catalog # MAB1849

Clone 210845 was used by HLDA to establish CD designation
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Insect, Moth - Trichoplusia ni (Cabbage Looper)

Applications

Validated:

Flow Cytometry, Agonist Activity, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development, Epitope Mapping

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 210845
View all NKp30/NCR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse T cell hybridoma transfected with human NKp30/NCR3 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera

Specificity

Detects human NKp30/NCR3 on cell transfectants and peripheral blood NK cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human NKp30/NCR3 Antibody

Human NKp30 Antibody Induces IFN-? Secretion antibody in NK-92 Cells.

Human NKp30 Antibody Induces IFN‑ gamma Secretion in NK‑92 Cells.

Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Human IFN-gamma DuoSet ELISA Kit (Catalog # DY285B). The ED50 for this effect is typically 0.2-1.2 µg/mL.
Detection of NKp30/NCR3 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of NKp30/NCR3 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with either (A) Mouse Anti-Human NKp30/NCR3 Monoclonal Antibody (Catalog # MAB1849) or (B) Mouse IgG2A Isotype Control (MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB2408P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of NKp30/NCR3 by Flow Cytometry

Detection of NKp30/NCR3 by Flow Cytometry

beta -1,3-glucan is required for NKp30-mediated killing of Cryptococcus. g NKp30 binding to C. neoformans vs. C. albicans analyzed using flow cytometry. The experiment was performed twice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NKp30/NCR3 by Flow Cytometry

Detection of NKp30/NCR3 by Flow Cytometry

beta -1,3-glucan is required for NKp30-mediated killing of Cryptococcus. f Anti-NKp30 mAb (1C01) blocked NKp30 binding to beta -1,3-GB. Recombinant NKp30 was incubated with 1C01 before being applied to beads conjugated with beta -1,3-glucan. The presence of NKp30 on beta -1,3-GB was detected using the polyclonal anti-NKp30 antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NKp30/NCR3 by Western Blot

Detection of NKp30/NCR3 by Western Blot

beta -1,3-glucan increases expression & clustering of NKp30 in NK. a NKp30 expression in YT in response to beta -1,3-glucan. YT treated with beta -1,3-glucan (III & IV) & expression of NKp30 using 1C01 was compared to untreated (I), or untreated labeled with isotype control (II). b WB analysis of NKp30 expression in YT in response to beta -1,3-glucan. YT incubated with or without beta -1,3-glucan for various times. The experiment was repeated three times with similar results. c NKp30 labeling at the synapse between a representative single YT cell & a single beta -1,3-glucan-conjugated bead. 1C01 was used to label NKp30. The location of the bead is shown with a white arrow. Bar = 5 µm. d Proximity of NKp30 expression in relation to the radius defined by the center of the IS (n = 21 ) in panel c. The method for determining the angle is illustrated in Supplementary Fig. 6C. Clustering was defined when the peak of fluorescence was at an angle <45° (blue bars in the left). e Frequency of NKp30 clustering of all YT cell conjugates with beta -1,3-glucan-conjugated or unconjugated beads as analyzed in panel c, as defined in panel d. f TIRF microscopic analysis of NKp30 expression at the interface between YT & beta -1,3-glucan or control. YT loaded into a glass chamber that had been coated with beta -1,3-glucan or mannan incubated at 37 °C for 30 min, fixed & stained for NKp30 using 1C01. Bar = 10 µm. g Fluorescent intensity of NKp30 from panel f in arbitrary units. Distance is defined in Supplementary Fig. 6A-B. All experiments repeated three times with similar results. TIRF total internal reflection fluorescence, poly-l-lysine coating alone or mannan coating served as control, Au arbitrary unit, Arrow bead, SD spinning disc microscopy, DIC differential interference contrast digital image, IS immunological synapse Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NKp30/NCR3 by Flow Cytometry

Detection of NKp30/NCR3 by Flow Cytometry

beta -1,3-glucan is required for NKp30-mediated killing of Cryptococcus. e Flow cytometric analysis of a recombinant NKp30-Fc fusion protein binding to beta -glucan-conjugated beads ( beta -1,3-GB) compared to unconjugated polystyrene beads as control. NKp30-Fc on the beads was detected with anti-NKp30 antibody (1C01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human NKp30/NCR3 Antibody

Application
Recommended Usage

Agonist Activity

0.2-1.2 µg/mL
Sample: NK-92 human natural killer lymphoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood lymphocytes

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

References

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.

Alternate Names

CD337, NCR3

Entrez Gene IDs

259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)

Gene Symbol

NCR3

Additional NKp30/NCR3 Products

Product Documents for Human NKp30/NCR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NKp30/NCR3 Antibody

For research use only

Citations for Human NKp30/NCR3 Antibody

Customer Reviews for Human NKp30/NCR3 Antibody

There are currently no reviews for this product. Be the first to review Human NKp30/NCR3 Antibody and earn rewards!

Have you used Human NKp30/NCR3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies