Human NKp30/NCR3 Antibody

Clone 210845 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
Human NKp30 Antibody Induces IFN‑ gamma  Secretion in NK‑92 Cells.
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Product Details
Citations (7)
Supplemental Products

Human NKp30/NCR3 Antibody Summary

Species Reactivity
Detects human NKp30/NCR3 on cell transfectants and peripheral blood NK cells.
Monoclonal Mouse IgG2A Clone # 210845
Protein A or G purified from hybridoma culture supernatant
Mouse T cell hybridoma transfected with human NKp30/NCR3 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

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Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
Human whole blood lymphocytes
Agonist Activity
0.2-1.2 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity Human NKp30 Antibody Induces IFN-? Secretion antibody in NK-92 Cells. View Larger

Human NKp30 Antibody Induces IFN‑ gamma Secretion in NK‑92 Cells. Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Human IFN-gamma DuoSet ELISA Kit (Catalog # DY285B). The ED50 for this effect is typically 0.2-1.2 µg/mL.

Flow Cytometry Detection of NKp30/NCR3 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of NKp30/NCR3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with either (A) Mouse Anti-Human NKp30/NCR3 Monoclonal Antibody (Catalog # MAB1849) or (B) Mouse IgG2A Isotype Control (MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB2408P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Entrez Gene IDs
259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)
Alternate Names
Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS

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Citations for Human NKp30/NCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. PVR and ICAM-1 on Blast Crisis CML Stem and Progenitor Cells with TKI Resistance Confer Susceptibility to NK Cells
    Authors: N Kim, MY Kim, YU Cho, W Chen, KH Lee, HS Kim
    Cancers (Basel), 2020;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells
    Authors: Z Djaoud, LA Guethlein, A Horowitz, T Azzi, N Nemat-Gorg, D Olive, D Nadal, PJ Norman, C Münz, P Parham
    J. Exp. Med., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
    Authors: Herrmann, Julia, Berberich, Hannah, Hartmann, Jessica, Beyer, Steffen, Davies, Karen, Koch, Joachim
    J Biol Chem, 2014;289(2):765-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Epitope Mapping
  7. Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan.
    Authors: Warren HS, Jones AL, Freeman C, Bettadapura J, Parish CR
    J. Immunol., 2005;175(1):207-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Mouse IgG2A Isotype Control

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