Human NKp30/NCR3 Antibody

Clone 210845 was used by HLDA to establish CD designation.
(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NKp30/NCR3 on cell transfectants and peripheral blood NK cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 210845
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse T cell hybridoma transfected with human NKp30/NCR3 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood CD56+ natural killer cells
  • Agonist Activity
    0.2-1.2 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Human NKp30 Antibody Induces IFN‑ gamma  Secretion in NK‑92 Cells.

Human NKp30 Monoclonal Antibody induces IFN‑ gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Quantikine Human IFN‑ gamma ELISA Kit (Catalog # DIF50). The ED50 for this effect is typically 0.2‑1.2 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
    4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
  • Entrez Gene IDs:
    259197 (Human); 294251 (Rat)
  • Alternate Names:
    Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
    Authors: Herrmann, Julia, Berberich, Hannah, Hartmann, Jessica, Beyer, Steffen, Davies, Karen, Koch, Joachim
    J Biol Chem, 2014;289(2):765-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
    Authors: Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F
    Blood, 2007;111(3):1309-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Type: Complex Sample Type
    Application: Epitope Mapping
  4. Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan.
    Authors: Warren HS, Jones AL, Freeman C, Bettadapura J, Parish CR
    J. Immunol., 2005;175(1):207-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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