Human NKp30/NCR3 Antibody

Human NKp30 Antibody Induces IFN‑ gamma Secretion in NK‑92 Cells. Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Human IFN-gamma DuoSet ELISA Kit (Catalog # DY285B). The ED₅₀ for this effect is typically 0.2-1.2 µg/mL.

Detection of NKp30/NCR3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with either (A) Mouse Anti-Human NKp30/NCR3 Monoclonal Antibody (Catalog # MAB1849) or (B) Mouse IgG2A Isotype Control (MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB2408P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of NKp30/NCR3 by Flow Cytometry beta -1,3-glucan is required for NKp30-mediated killing of Cryptococcus. g NKp30 binding to C. neoformans vs. C. albicans analyzed using flow cytometry. The experiment was performed twice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of NKp30/NCR3 by Flow Cytometry beta -1,3-glucan is required for NKp30-mediated killing of Cryptococcus. f Anti-NKp30 mAb (1C01) blocked NKp30 binding to beta -1,3-GB. Recombinant NKp30 was incubated with 1C01 before being applied to beads conjugated with beta -1,3-glucan. The presence of NKp30 on beta -1,3-GB was detected using the polyclonal anti-NKp30 antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of NKp30/NCR3 by Western Blot beta -1,3-glucan increases expression & clustering of NKp30 in NK. a NKp30 expression in YT in response to beta -1,3-glucan. YT treated with beta -1,3-glucan (III & IV) & expression of NKp30 using 1C01 was compared to untreated (I), or untreated labeled with isotype control (II). b WB analysis of NKp30 expression in YT in response to beta -1,3-glucan. YT incubated with or without beta -1,3-glucan for various times. The experiment was repeated three times with similar results. c NKp30 labeling at the synapse between a representative single YT cell & a single beta -1,3-glucan-conjugated bead. 1C01 was used to label NKp30. The location of the bead is shown with a white arrow. Bar = 5 µm. d Proximity of NKp30 expression in relation to the radius defined by the center of the IS (n = 21 ) in panel c. The method for determining the angle is illustrated in Supplementary Fig. 6C. Clustering was defined when the peak of fluorescence was at an angle <45° (blue bars in the left). e Frequency of NKp30 clustering of all YT cell conjugates with beta -1,3-glucan-conjugated or unconjugated beads as analyzed in panel c, as defined in panel d. f TIRF microscopic analysis of NKp30 expression at the interface between YT & beta -1,3-glucan or control. YT loaded into a glass chamber that had been coated with beta -1,3-glucan or mannan incubated at 37 °C for 30 min, fixed & stained for NKp30 using 1C01. Bar = 10 µm. g Fluorescent intensity of NKp30 from panel f in arbitrary units. Distance is defined in Supplementary Fig. 6A-B. All experiments repeated three times with similar results. TIRF total internal reflection fluorescence, poly-l-lysine coating alone or mannan coating served as control, Au arbitrary unit, Arrow bead, SD spinning disc microscopy, DIC differential interference contrast digital image, IS immunological synapse Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of NKp30/NCR3 by Flow Cytometry beta -1,3-glucan is required for NKp30-mediated killing of Cryptococcus. e Flow cytometric analysis of a recombinant NKp30-Fc fusion protein binding to beta -glucan-conjugated beads ( beta -1,3-GB) compared to unconjugated polystyrene beads as control. NKp30-Fc on the beads was detected with anti-NKp30 antibody (1C01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29467448), licensed under a CC-BY license. Not internally tested by R&D Systems.