Detects human Notch-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity
with recombinant human (rh) Notch-1 ICD, rhNotch-3 ECD, rhNotch-4 ICD,
recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, or rrDelta-1 is observed.
Monoclonal Mouse IgG1 Clone # 602845
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Notch-2 Leu26-Gln530 Accession # Q04721
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Notch‑2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Notch‑2 APC‑conjugated Monoclonal Antibody (Catalog # FAB37351A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.