Human Notch-2 APC-conjugated Antibody Summary
Accession # Q04721
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Notch‑2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Notch-2 APC-conjugated Monoclonal Antibody (Catalog # FAB37351A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.
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