Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 602845
View all Notch-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Notch-2
Leu26-Gln530
Accession # Q04721

Specificity

Detects human Notch-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Notch-1 ICD, rhNotch-3 ECD, rhNotch-4 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, or rrDelta-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Notch‑2 APC‑conjugated Antibody

Detection of Notch-2 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry.

Detection of Notch‑2 in NTera‑2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Notch-2 APC-conjugated Monoclonal Antibody (Catalog # FAB37351A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Notch‑2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Notch-2

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Gene Symbol

NOTCH2

UniProt

Q04721

Additional Notch-2 Products

Product Documents for Human Notch‑2 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Notch‑2 APC‑conjugated Antibody

For research use only

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