Human Notch-2 Antibody

Catalog #: MAB37351
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB37351
MAB37351-SP

Detection of Notch‑2 in NTera-2 Human Cell Line by Flow Cytometry.

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All Notch-2 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Notch-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Notch-1 ICD, rhNotch-3 ECD, rhNotch-4 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, or rrDelta-1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 602845

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Notch-2
Leu26-Gln530
Accession # Q04721

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Notch‑2 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Human Notch-2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Flow Cytometry

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Detection of Notch‑2 in Raji Human Cell Line by Flow Cytometry. Raji Burkit lymphoma human cell line was stained with Human Notch-2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-2

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Alternate Names

AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Notch-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Dysregulated Notch Signaling in the Airway Epithelium of Children with Wheeze
Authors: T Iosifidis, EN Sutanto, ST Montgomery, P Agudelo-Ro, K Looi, KM Ling, NC Shaw, LW Garratt, J Hillas, KM Martinovic, E Kicic-Star, S Vijayaseka, FJ Lannigan, PJ Rigby, DA Knight, SM Stick, A Kicic
Journal of personalized medicine, 2021-12-07;11(12):.
Species: Human
Sample Types: Whole Cells
Applications: In-Cell Western