Human Notch-2 Antibody Summary
Accession # Q04721
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Notch‑2 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Human Notch-2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Detection of Notch‑2 in Raji Human Cell Line by Flow Cytometry. Raji Burkit lymphoma human cell line was stained with Human Notch-2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.
Citation for Human Notch-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Dysregulated Notch Signaling in the Airway Epithelium of Children with Wheeze
Authors: T Iosifidis, EN Sutanto, ST Montgomery, P Agudelo-Ro, K Looi, KM Ling, NC Shaw, LW Garratt, J Hillas, KM Martinovic, E Kicic-Star, S Vijayaseka, FJ Lannigan, PJ Rigby, DA Knight, SM Stick, A Kicic
Journal of personalized medicine, 2021;11(12):.
Sample Types: Whole Cells
Applications: In-Cell Western
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