Detects human Notch-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity
with recombinant human (rh) Notch-1 ICD, rhNotch-3 ECD, rhNotch-4 ICD,
recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, or rrDelta-1 is observed.
Monoclonal Mouse IgG1 Clone # 602845
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Notch-2 Leu26-Gln530 Accession # Q04721
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Notch‑2 in NTera-2 Human Cell Line by Flow Cytometry.
NTera‑2 human testicular embryonic carcinoma cell line was stained with Human Notch‑2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Detection of Notch‑2 in Raji Human Cell Line by Flow Cytometry.
Raji Burkit lymphoma human cell line was stained with Human Notch‑2 Monoclonal Antibody (Catalog # MAB37351, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins. The 2446 amino acid (aa) type I TM glycoprotein undergoes Golgi processing to generate a heterodimer of 180 kDa disulfide-linked extracellular domain (ECD) and 110 kDa membrane bound segments. Binding of Notch ligands, including Jagged and Delta-like molecules, has been localized to the 11th and 12th EGF-like repeats, aa 415-492. Human Notch-2 ECD (aa 26-530) shares 93% aa identity with mouse or rat Notch-2 ECD.
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