Detects human Notch-3 in direct ELISAs and Western blots. In direct ELISAs and Western blot, approximately 30% cross-reactivity with recombinant mouse Notch-3 is observed and less than 5% cross-reactivity with recombinant rat Notch-2 is observed.
Polyclonal Sheep IgG
Mouse myeloma cell line NS0-derived recombinant human Notch-3 Ala40-Glu467 Accession # Q9UM47
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Notch‑3 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Sheep Anti-Human Notch‑3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1559A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes (1). The extracellular domain of Notch receptors interact with the extracellular domain of the transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of neighboring cells. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The Notch molecule is synthesized as a 2321amino acid (aa) precursor that contains an 39 aa signal sequence, a 1603 aa extracellular region, a 21aa transmembrane (TM) segment and a 658 aa cytoplasmic domain. The large Notch extracellular domain has 34 EGF-like repeats followed by three notch/Lin-12 repeats (LNR) (2). The 11th and 12th EGF-like repeats of Notch have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta in Drosophila (3). Notch-3 has the same biochemical mechanism of signal tranduction as Notch-1, where a series of cleavage events result in the release of the Notch Intracellular Domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus, Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription. Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia. Nearly all mutations leading to this disorder are clustered in the first 5 EGF repeats of the Notch-3 gene (5). Human Notch-3 shows 90% aa sequence identity to mouse Notch-3 over the entire protein.
Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
Joutel, A. et al. (1996) Nature 383:707.
Rebay, I. et al. (1991) Cell 67:687.
Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.
Joutel, A. and E. Tournier-Lasserve (1998) Semin. Cell Dev. Biol. 9:619.
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