Human Notch-3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Notch-3 in direct ELISAs and Western blots. In direct ELISAs and Western blot, approximately 30% cross-reactivity with recombinant mouse Notch-3 is observed and less than 5% cross-reactivity with recombinant rat Notch-2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Notch-3
    Accession # Q9UM47
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Notch‑3 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Sheep Anti-Human Notch‑3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1559A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Notch-3

Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes (1). The extracellular domain of Notch receptors interact with the extracellular domain of  the transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of neighboring cells. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The Notch molecule is synthesized as a 2321amino acid (aa) precursor that contains an 39 aa signal sequence, a 1603 aa extracellular region, a 21aa transmembrane (TM) segment and a 658 aa cytoplasmic domain. The large Notch extracellular domain has 34 EGF-like repeats followed by three notch/Lin-12 repeats (LNR) (2). The 11th and 12th EGF-like repeats of Notch have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta in Drosophila (3). Notch-3 has the same biochemical mechanism of signal tranduction as Notch-1, where a series of cleavage events result in the release of the Notch Intracellular Domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus, Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription. Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia. Nearly all mutations leading to this disorder are clustered in the first 5 EGF repeats of the Notch-3 gene (5). Human Notch-3 shows 90% aa sequence identity to mouse Notch-3 over the entire protein.

  • References:
    1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
    2. Joutel, A. et al. (1996) Nature 383:707.
    3. Rebay, I. et al. (1991) Cell 67:687.
    4. Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.
    5. Joutel, A. and E. Tournier-Lasserve (1998) Semin. Cell Dev. Biol. 9:619.
  • Entrez Gene IDs:
    4854 (Human); 18131 (Mouse)
  • Alternate Names:
    CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG APC-conjugated Antibody

Ctrl IC016A 1  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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