Human OBCAM/OPCML Antibody

Catalog # Availability Size / Price Qty
MAB27771
MAB27771-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human OBCAM/OPCML Antibody Summary

Species Reactivity
Human
Specificity
Detects human OBCAM on direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM, recombinant mouse (rm) MAdCAM, rmOCAM, or rmNCAM-L1.
Source
Monoclonal Mouse IgG1 Clone # 341723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human OBCAM
Gly28-Asn322
Accession # Q14982
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human OBCAM (Catalog # 2777-CM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OBCAM/OPCML

OBCAM (Opioid-binding cell adhesion molecule, also known as OPCML) is a member of the IgLON family of cell adhesion molecules. All IgLONs are GPI-linked glycoproteins, contain three C2 type Ig-like domains, and are expressed in cerebral cortex and hippocampus (1, 2). The name IgLON derives from family membership in the Ig superfamily, and the first letters of the names of group’s molecules; LAMP, OBCAM, and Neurotrimin. Recently, membership in the group has been expanded by one with the addition of Kilon (Kindred of IgLON), and members of this group are now often referred to Diglons, based on the dimerizing nature of the IgLONs (1, 2). Human OBCAM is synthesized as a 345 amino acid (aa) preproprecursor that contains a 27 aa signal sequence, a 295 aa mature region, and a
C‑terminal 23 aa prosegment (3). The prosegment is cleaved to generate the GPI-link. OBCAM varies in molecular weight, ranging from 46 kDa to 65 kDa (4 - 6). The difference is not due to alternate splicing but to differential glycosylation (6). Although it is not unusual for GPI-linked proteins to be solubilized, to date there is no evidence that OBCAM functions as a soluble molecule (1). Mature human OBCAM is 98%, 99%, and 98% aa identical to mature bovine, rat and mouse OBCAM, respectively. OBCAM has limited expression, occuring principally in telencephalon and ovarian epithelium (7, 8). In brain, it is found associated with dendrites and post-synaptic membranes, where it may maintain synaptic architecture (1, 5). In ovary, it has been suggested to be a tumor-suppressor factor (8). The receptor(s) for OBCAM appears to be other members of the IgLON family, and a dimer is the functional unit. While neurotrimin appears to function as both a homodimer and heterodimer, all other family members (including OBCAM) show a preference for heterodimerization. OBCAM forms strong trans (between cells) heterodimers with LAMP, and modest heterodimers with Neurotrimin. There is hardly any binding with itself. Kilon likely binds OBCAM, but this interaction is not well studied (1). OBCAM heterodimers apparently bind to almost all possible IgLON heterodimer combinations on other cells. In cis (same cell), OBCAM also binds to LAMP and Neurotrimin (2).

References
  1. Miyata, S. et al. (2000) Neuroscience 117:645.
  2. Reed, J. et al. (2004) J. Cell Sci. 117:3961.
  3. Shark, K.B. and N.M. Lee (1995) Gene 155:213.
  4. Wick, M.J. et al. (1996) Mol. Brain Res. 36:322.
  5. Miyata, S. (2000) J. Comp. Neurol. 242:74.
  6. Hachisuka, A. et al. (1996) Neurochem. Int. 28:373.
  7. Miyata, S. et al. (2003) Brain Res. 979:129.
  8. Sellar, G.C. et al. (2003) Nat. Genet. 34:337.
Long Name
Opioid-binding Cell Adhesion Molecule/Opioid Binding Protein/Cell Adhesion Molecule-like
Entrez Gene IDs
4978 (Human); 330908 (Mouse)
Alternate Names
IgLON family member 1; IGLON1opioid-binding protein/cell adhesion molecule-like; OBCAM; OBCAMopiate binding-cell adhesion molecule; OPCM; OPCML; opioid binding protein/cell adhesion molecule-like preprotein; opioid binding protein/cell adhesion molecule-like; Opioid-binding cell adhesion molecule; opioid-binding protein/cell adhesion molecule

Product Datasheets

Citations for Human OBCAM/OPCML Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions
    Authors: JR Birtley, M Alomary, E Zanini, J Antony, Z Maben, GC Weaver, C Von Arx, M Mura, AT Marinho, H Lu, EVN Morecroft, E Karali, NE Chayen, EW Tate, M Jurewicz, LJ Stern, C Recchi, H Gabra
    Nat Commun, 2019;10(1):3134.
    Species: Human
    Sample Types: Whole Cells
    Applications: Fluorescence Microscopy
  2. CpG-island methylation study of liver fluke-related cholangiocarcinoma.
    Authors: Sriraksa R, Zeller C, El-Bahrawy MA
    Br. J. Cancer, 2011;104(8):1313-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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