Human Otx2 Biotinylated Antibody

  (13 citations)     
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Product Details
Citations (13)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Otx2 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Otx2 Met1-Leu289 Accession # P32243
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Otx2
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunocytochemistry
Otx2 in BG01V Human Embryonic Stem Cells. Otx2 was detected in immersion fixed BG01V human embryonic stem cells differentiated to ectoderm using Goat Anti-Human Otx2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated AntI-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
      12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Otx2
Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.
  • References:
    1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
  • Long Name:
    Orthodenticle Homolog 2
  • Entrez Gene IDs:
    5015 (Human)
  • Alternate Names:
    homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina
    Authors: KU Park, G Randazzo, KL Jones, JA Brzezinski
    Invest. Ophthalmol. Vis. Sci, 2017;58(2):1137-1150.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  2. Arap1 Deficiency Causes Photoreceptor Degeneration in Mice
    Authors: A Moshiri, D Humpal, BC Leonard, DM Imai, A Tham, L Bower, D Clary, TM Glaser, KC Lloyd, CJ Murphy
    Invest. Ophthalmol. Vis. Sci, 2017;58(3):1709-1718.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa
    Authors: TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone
    Stem Cell Res, 2017;21(0):58-70.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Combinatorial regulation of a Blimp1 (Prdm1) enhancer in the mouse retina
    Authors: TS Mills, T Eliseeva, SM Bersie, G Randazzo, J Nahreini, KU Park, JA Brzezinski
    PLoS ONE, 2017;12(8):e0176905.
    Species: Mouse
    Sample Type: Protein
    Application: WB
  5. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
    Sci Rep, 2016;6(0):30742.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Fresh
  6. The GIPC1-Akt1 Pathway Is Required for the Specification of the Eye Field in Mouse Embryonic Stem Cells.
    Authors: La Torre A, Hoshino A, Cavanaugh C, Ware C, Reh T
    Stem Cells, 2015;33(9):2674-85.
    Species: Mouse
    Sample Type: Whole Organism
    Application: IHC Not Specified
  7. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation.
    Authors: Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T
    Development, 2015;142(3):533-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. Stage-dependent requirement of neuroretinal Pax6 for lens and retina development.
    Authors: Klimova, Lucie, Kozmik, Zbynek
    Development, 2014;141(6):1292-302.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence.
    Authors: Brzezinski J, Uoon Park K, Reh T
    Dev Biol, 2013;384(2):194-204.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  10. Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation.
    Authors: Tucker B, Anfinson K, Mullins R, Stone E, Young M
    Stem Cells Transl Med, 2013;2(1):16-24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  11. Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region.
    Authors: Achim K, Peltopuro P, Lahti L
    Development, 2012;139(13):2360-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. OTX2 is critical for the maintenance and progression of Shh-independent medulloblastomas.
    Authors: Adamson DC, Shi Q, Wortham M
    Cancer Res., 2010;70(1):181-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development.
    Authors: Brzezinski JA, Lamba DA, Reh TA
    Development, 2010;137(4):619-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all Citations

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Isotype Controls
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Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Staining Reagents
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
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Streptavidin-Phycoerythrin

Flow F0040 7
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NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
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Streptavidin-Allophycocyanin

Flow F0050 1
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NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
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NorthernLights Streptavidin NL637

Flow , IHC , ICC NL998 2
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Avidin-Fluorescein

Flow F0030 1
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 51  
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EvenCoat Streptavidin Coated Plates

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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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