Human Otx2 Antibody [Biotin]

R&D Systems | Catalog # BAF1979

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CUT&Tag

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Otx2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243

Specificity

Detects human Otx2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Otx2 Antibody [Biotin]

Otx2 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Otx2 in BG01V Human Embryonic Stem Cells.

Otx2 was detected in immersion fixed BG01V human embryonic stem cells differentiated to ectoderm using Goat Anti-Human Otx2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated AntI-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Otx2 Antibody [Biotin]

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Otx2

Reviewed Applications

Read 1 review rated 4 using BAF1979 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References

  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Long Name

Orthodenticle Homolog 2

Alternate Names

homeobox protein OTX2, MCOPS5, MGC45000, orthodenticle homeobox 2, Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), OTX2

Entrez Gene IDs

5015 (Human)

Gene Symbol

OTX2

UniProt

P32243

Additional Otx2 Products

Product Documents for Human Otx2 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Otx2 Antibody [Biotin]

For research use only

Citations for Human Otx2 Antibody [Biotin]

Customer Reviews for Human Otx2 Antibody [Biotin] (1)

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  • Human Otx2 Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic retina
    Species: Mouse
    Verified Customer | Posted 10/13/2018

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Protocols

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