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Human Otx2 Biotinylated Antibody

Catalog #: BAF1979
20 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF1979
Otx2 in BG01V Human Embryonic Stem Cells.
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Citations (20)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Otx2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Otx2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Otx2
Met1-Leu289
Accession # P32243
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Otx2
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Otx2 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Otx2 in BG01V Human Embryonic Stem Cells. Otx2 was detected in immersion fixed BG01V human embryonic stem cells differentiated to ectoderm using Goat Anti-Human Otx2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated AntI-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otx2

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

References
  1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.
Long Name
Orthodenticle Homolog 2
Entrez Gene IDs
5015 (Human)
Alternate Names
homeobox protein OTX2; MCOPS5; MGC45000; orthodenticle homeobox 2; orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Otx2

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Citations for Human Otx2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Single-cell ATAC-seq of fetal human retina and stem-cell-derived retinal organoids shows changing chromatin landscapes during cell fate acquisition
    Authors: C Finkbeiner, I Ortuño-Liz, A Sridhar, M Hooper, S Petter, TA Reh
    Cell Reports, 2022;38(4):110294.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Efficient stimulation of retinal regeneration from M�ller glia in adult mice using combinations of proneural bHLH transcription factors
    Authors: L Todd, MJ Hooper, AK Haugan, C Finkbeiner, N Jorstad, N Radulovich, CK Wong, PC Donaldson, W Jenkins, Q Chen, F Rieke, TA Reh
    Cell Reports, 2021;37(3):109857.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  3. Microglia Suppress Ascl1-Induced Retinal Regeneration in Mice
    Authors: L Todd, C Finkbeiner, CK Wong, MJ Hooper, TA Reh
    Cell Reports, 2020;33(11):108507.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures
    Authors: A Sridhar, A Hoshino, CR Finkbeiner, A Chitsazan, L Dai, AK Haugan, KM Eschenbach, DL Jackson, C Trapnell, O Bermingham, I Glass, TA Reh
    Cell Rep, 2020;30(5):1644-1659.e4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. STAT Signaling Modifies Ascl1 Chromatin Binding and Limits Neural Regeneration from Muller Glia in Adult Mouse Retina
    Authors: NL Jorstad, MS Wilken, L Todd, C Finkbeiner, P Nakamura, N Radulovich, MJ Hooper, A Chitsazan, BA Wilkerson, F Rieke, TA Reh
    Cell Rep, 2020;30(7):2195-2208.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Synchrony and asynchrony between an epigenetic clock and developmental timing
    Authors: A Hoshino, S Horvath, A Sridhar, A Chitsazan, TA Reh
    Sci Rep, 2019;9(1):3770.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  7. RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion
    Authors: CL Fairchild, K Hino, JS Han, AM Miltner, GP Allina, CE Brown, ME Burns, A La Torre, S Simo
    Development, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina
    Authors: KU Park, G Randazzo, KL Jones, JA Brzezinski
    Invest. Ophthalmol. Vis. Sci, 2017;58(2):1137-1150.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Combinatorial regulation of a Blimp1 (Prdm1) enhancer in the mouse retina
    Authors: TS Mills, T Eliseeva, SM Bersie, G Randazzo, J Nahreini, KU Park, JA Brzezinski
    PLoS ONE, 2017;12(8):e0176905.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  10. Arap1 Deficiency Causes Photoreceptor Degeneration in Mice
    Authors: A Moshiri, D Humpal, BC Leonard, DM Imai, A Tham, L Bower, D Clary, TM Glaser, KC Lloyd, CJ Murphy
    Invest. Ophthalmol. Vis. Sci, 2017;58(3):1709-1718.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa
    Authors: TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone
    Stem Cell Res, 2017;21(0):58-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness
    Sci Rep, 2016;6(0):30742.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation.
    Authors: Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T
    Development, 2015;142(3):533-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. The GIPC1-Akt1 Pathway Is Required for the Specification of the Eye Field in Mouse Embryonic Stem Cells.
    Authors: La Torre A, Hoshino A, Cavanaugh C, Ware C, Reh T
    Stem Cells, 2015;33(9):2674-85.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: IHC
  15. Stage-dependent requirement of neuroretinal Pax6 for lens and retina development.
    Authors: Klimova, Lucie, Kozmik, Zbynek
    Development, 2014;141(6):1292-302.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence.
    Authors: Brzezinski J, Uoon Park K, Reh T
    Dev Biol, 2013;384(2):194-204.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  17. Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation.
    Authors: Tucker B, Anfinson K, Mullins R, Stone E, Young M
    Stem Cells Transl Med, 2013;2(1):16-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region.
    Authors: Achim K, Peltopuro P, Lahti L
    Development, 2012;139(13):2360-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development.
    Authors: Brzezinski JA, Lamba DA, Reh TA
    Development, 2010;137(4):619-29.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. OTX2 is critical for the maintenance and progression of Shh-independent medulloblastomas.
    Authors: Adamson DC, Shi Q, Wortham M
    Cancer Res., 2010;70(1):181-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Otx2 Biotinylated Antibody
By Anonymous on 10/13/2018
Application: ICC/IF Sample Tested: Embryonic retina Species: Mouse