The E1A-binding protein p300 is a histone acetyltransferase that also acts to acetylate other proteins, such as p53. p300 acts as a transcriptional coactivator that can serve as an adaptor molecule to bridge to transcriptional regulators. In addition, p300 binds to PCNA and may participate in chromatin remodeling.
Human p300 Biotinylated Antibody
R&D Systems | Catalog # BAF3789
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Proximity Ligation Assay (PLA)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human p300
His2283-His2414
Accession # Q09472
His2283-His2414
Accession # Q09472
Specificity
Detects human p300 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human p300 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human p300
Sample: Recombinant Human p300
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: p300
Long Name
E1A-binding Protein p300
Alternate Names
EP300
Gene Symbol
EP300
UniProt
Additional p300 Products
Product Documents for Human p300 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human p300 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Human p300 Biotinylated Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars