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Human PARP Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB8095T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB8095T-100UG

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Human PARP Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PARP in ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 839120
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human PARP
Thr373-Glu540
Accession # P09874
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Knockout Validated
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PARP

PARP, Poly [ADP-ribose] polymerase 1 (PARP1), is a widely expressed component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. PARP expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult. PARP is upregulated in B cells that have been induced to switch to various Ig isotypes. PARP interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression.

Long Name
Poly [ADP-ribose] Polymerase
Entrez Gene IDs
142 (Human); 11545 (Mouse)
Alternate Names
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1

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