Human PBEF/Visfatin Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB4335G-100UG

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Human PBEF/Visfatin Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PBEF/Visfatin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 882115R
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant Human PBEF/Visfatin
Pro27-His491
Accession # P43490
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
ELISA
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name
Pre-B-Cell Colony-Enhancing Factor 1
Entrez Gene IDs
10135 (Human); 59027 (Mouse); 297508 (Rat)
Alternate Names
EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin

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