Human PBEF/Visfatin Antibody

Recombinant Monoclonal Antibody
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Human PBEF/Visfatin ELISA Standard Curve.
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Product Details
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Human PBEF/Visfatin Antibody Summary

Species Reactivity
Detects human PBEF/Visfatin in direct ELISAs.
Recombinant Monoclonal Mouse IgG1 Clone # 882115R
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant Human PBEF/Visfatin
Accession # P43490
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PBEF/Visfatin DuoSet ELISA Kit (Catalog # DY4335-05) for convenient development of a sandwich ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human PBEF/Visfatin ELISA Standard Curve. Recombinant Human PBEF/Visfatin protein was serially diluted 2-fold and captured by Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name
Pre-B-Cell Colony-Enhancing Factor 1
Entrez Gene IDs
10135 (Human); 59027 (Mouse); 297508 (Rat)
Alternate Names
EC; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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