Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
R&D Systems | Catalog # MAB10348
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu124
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Product Specifications
Immunogen
Human PD-L1/B7-H1
Specificity
Detects Human PD-L1/B7-H1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Atezolizumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
Detection of PD-L1/B7-H1 in MDA-MB-231 human breast adenocarcinoma cell line by Flow Cytometry.
MDA-MB-231 human breast adenocarcinoma cell line was stained with Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (F0135). View our protocol for Staining Membrane-associated Proteins.
PD-1 binding to Human PD-L1/B7-H1-transfected HEK293 Human Cell Line is Blocked by Human PD-L1/B7-H1 Antibody.
In a functional flow cytometry test, Recombinant Human PD-1 His-tagged protein (8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348) but not by (B) Human IgG Isotype Control (1-001-A). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC050A).Applications for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, 2.5 μg/mL of Human anti-human PD-L1/B7-H1 (Atezoliumab Biosimilar) Antibody(Catalog # MAB10348) will block the binding of Recombinant Human PD-1 His-tagged protein (Catalog # 8986-PD) to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: MDA-MB-231 human breast adenocarcinoma cell line
Sample: MDA-MB-231 human breast adenocarcinoma cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PD-L1/B7-H1
Long Name
Programmed Death Ligand 1
Alternate Names
B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Entrez Gene IDs
Gene Symbol
CD274
Additional PD-L1/B7-H1 Products
Product Documents for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
For research use only
Citations for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody
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Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.
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Q: Is the Fc portion of Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody, Catalog # MAB10348, inert, similar to what has been reported with the therapeutic Atezolizumab?
A: We have not performed any testing in house to confirm that the Fc portion is inert, but the sequence of MAB10348 is the same as the therapeutic Atezolizumab.
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Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.
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Q: Is the Fc portion of Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody, Catalog # MAB10348, inert, similar to what has been reported with the therapeutic Atezolizumab?
A: We have not performed any testing in house to confirm that the Fc portion is inert, but the sequence of MAB10348 is the same as the therapeutic Atezolizumab.
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