His Tag APC-conjugated Antibody

Catalog # Availability Size / Price Qty
His Tag APC-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Product Details
Citations (2)
Supplemental Products

His Tag APC-conjugated Antibody Summary

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
Monoclonal Mouse IgG1 Clone # AD1.1.10
Protein A or G purified from hybridoma culture supernatant
His-tagged peptide
Supplied in a saline solution containing BSA and Sodium Azide.


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. View Larger

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine

Product Datasheets

Citations for His Tag APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease
    Authors: A Cho, AL Caldara, NA Ran, Z Menne, RC Kauffman, M Affer, A Llovet, C Norwood, A Scanlan, G Mantus, B Bradley, S Zimmer, T Schmidt, M Hertl, AS Payne, R Feldman, AP Kowalczyk, J Wrammert
    Cell Rep, 2019;28(4):909-922.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers
    Authors: T Osada, ZC Hartman, J Wei, G Lei, AC Hobeika, WR Gwin, MA Diniz, N Spector, TM Clay, W Chen, MA Morse, HK Lyerly
    Breast Cancer Res., 2018;20(1):90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay


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