His Tag APC-conjugated Antibody
His Tag APC-conjugated Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody. (A) Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (Catalog # IC050A). (B) No staining was observed in the absence of protein. Staining was performed using our Staining Membrane-Associated Proteins protocol.
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Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
Product Datasheets
Citations for His Tag APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Successful targeting of PD-1/PD-L1 with chimeric antigen receptor-natural killer cells and nivolumab in a humanized mouse cancer model
Authors: WN Liu, WY So, SL Harden, SY Fong, MXY Wong, WWS Tan, SY Tan, JKL Ong, R Rajarethin, M Liu, JY Cheng, L Suteja, JPS Yeong, NG Iyer, DW Lim, Q Chen
Science Advances, 2022;8(47):eadd1187.
Species: Xenograft
Sample Types: Whole Cells
Applications: Flow Cytometry -
Single-Cell Analysis Suggests that Ongoing Affinity Maturation Drives the Emergence of Pemphigus Vulgaris Autoimmune Disease
Authors: A Cho, AL Caldara, NA Ran, Z Menne, RC Kauffman, M Affer, A Llovet, C Norwood, A Scanlan, G Mantus, B Bradley, S Zimmer, T Schmidt, M Hertl, AS Payne, R Feldman, AP Kowalczyk, J Wrammert
Cell Rep, 2019;28(4):909-922.e6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers
Authors: T Osada, ZC Hartman, J Wei, G Lei, AC Hobeika, WR Gwin, MA Diniz, N Spector, TM Clay, W Chen, MA Morse, HK Lyerly
Breast Cancer Res., 2018;20(1):90.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay
FAQs
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Staining Reagents
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