Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # PRa292
View all PDGF R alpha Primary Antibodies »

Product Specifications

Immunogen

Human osteosarcoma cell membrane extracts

Specificity

Detects human PDGF R alpha. Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PDGF R alpha PE‑conjugated Antibody

Detection of PDGF Ra antibody in U-118-MG Human Cell Line antibody by Flow Cytometry.

Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry.

U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1264P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PDGF R alpha PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line

Reviewed Applications

Read 1 review rated 4 using FAB1264P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References

  1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for Human PDGF R alpha PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PDGF R alpha PE‑conjugated Antibody

For research use only

Citations for Human PDGF R alpha PE‑conjugated Antibody

Customer Reviews for Human PDGF R alpha PE‑conjugated Antibody (1)

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  • Human PDGF R alpha PE-conjugated Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    We use to identify fibroadipogenic progenitors in skeletal muscle.

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