Human PDGF R alpha Antibody

Catalog # Availability Size / Price Qty
MAB1264
MAB1264-SP
Product Details
Citations (6)
FAQs
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Human PDGF R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R alpha. Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.
Source
Monoclonal Mouse IgG1 Clone # PRa292
Purification
Protein A or G purified from ascites
Immunogen
Human osteosarcoma cell membrane extracts
Formulation

Supplied as a 0.2 μm filtered solution in PBS.

*Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
U‑118‑MG human glioblastoma/astrocytoma cell line
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human PDGF R alpha (Catalog # 322‑PR), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Product Datasheets

Citations for Human PDGF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. PDGFRA alterations in cancer: characterization of a gain-of-function V536E transmembrane mutant as well as loss-of-function and passenger mutations.
    Authors: Velghe A, Van Cauwenberghe S, Polyansky A, Chand D, Montano-Almendras C, Charni S, Hallberg B, Essaghir A, Demoulin J
    Oncogene, 2014;33(20):2568-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Alveolar macrophages are critical for the inhibition of allergic asthma by mesenchymal stromal cells.
    Authors: Mathias, Louisa J, Khong, Sacha M, Spyroglou, Lisa, Payne, Natalie, Siatskas, Christop, Thorburn, Alison N, Boyd, Richard, Heng, Tracy S
    J Immunol, 2013;191(12):5914-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
    Authors: Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
    J. Immunol., 2007;179(7):4435-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
    Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
    Leuk. Lymphoma, 2007;48(10):2032-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  6. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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