Human PDGF R alpha Antibody

  (6 citations)     
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Citations (6)
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  • Species Reactivity
    Human
  • Specificity
    Detects human PDGF R alpha. Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.
  • Source
    Monoclonal Mouse IgG1 Clone # PRa292
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Human osteosarcoma cell membrane extracts
  • Formulation

    Supplied as a 0.2 μm filtered solution in PBS.

    *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

  • References:
    1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
  • Long Name:
    Platelet-derived Growth Factor Receptor alpha
  • Entrez Gene IDs:
    5156 (Human); 18595 (Mouse)
  • Alternate Names:
    alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. PDGFRA alterations in cancer: characterization of a gain-of-function V536E transmembrane mutant as well as loss-of-function and passenger mutations.
    Authors: Velghe A, Van Cauwenberghe S, Polyansky A, Chand D, Montano-Almendras C, Charni S, Hallberg B, Essaghir A, Demoulin J
    Oncogene, 2014;33(20):2568-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Alveolar macrophages are critical for the inhibition of allergic asthma by mesenchymal stromal cells.
    Authors: Mathias, Louisa J, Khong, Sacha M, Spyroglou, Lisa, Payne, Natalie, Siatskas, Christop, Thorburn, Alison N, Boyd, Richard, Heng, Tracy S
    J Immunol, 2013;191(12):5914-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Mesenchymal stem cell abnormalities in patients with multiple myeloma.
    Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
    Leuk. Lymphoma, 2007;48(10):2032-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
    Authors: Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
    J. Immunol., 2007;179(7):4435-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  6. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007;25(7):1737-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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